caa a22 4500 475746899 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/PL00021056 doi (NATIONALLICENCE)springer-10.1007/PL00021056 Characterization of the Alanine Racemases from Pseudomonas aeruginosa PAO1 [Elektronische Daten] [Ulrich Strych, Hung-Chung Huang, Kurt L. Krause, Michael J. Benedik] Alanine racemases are ubiquitous, almost uniquely prokaryotic enzymes catalyzing the racemization between l- and d-alanine. The requirement for d-alanine as a necessary component of the bacterial cell wall makes this class of enzymes a logical target for the development of novel antibiotics. In an effort to better understand the structure and mechanism of these enzymes, we have cloned the two independent alanine racemases from Pseudomonas aeruginosa, an important opportunistic bacterial pathogen of humans and animals. The dadX PA and alr PA genes have been sequenced, overexpressed, and their activity was demonstrated by complementing d-alanine auxotrophs of Escherichia coli. Both gene products were purified to electrophoretic homogeneity, the enzymes were characterized biochemically, and preliminary crystals were obtained. Springer-Verlag New York Inc., 2000 Strych Ulrich Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513, USA, US aut Huang Hung-Chung Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513, USA, US aut Krause Kurt L. Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513, USA, US aut Benedik Michael J. Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513, USA, US aut https://doi.org/10.1007/PL00021056 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00021056 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Strych Ulrich Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513, USA, US aut NATIONALLICENCE 700 1- Huang Hung-Chung Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513, USA, US aut NATIONALLICENCE 700 1- Krause Kurt L. Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513, USA, US aut NATIONALLICENCE 700 1- Benedik Michael J. Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer