caa a22 4500 475746902 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s002840010148 doi (NATIONALLICENCE)springer-10.1007/s002840010148 Analysis of Expression of the Binary Toxin Genes from Bacillus sphaericus in Anabaena and the Potential in Mosquito Control [Elektronische Daten] [Xudong Xu, Ge Yan, Renqiu Kong, Xiangping Liu, Lihong Yu] Anabaena strains expressing the binary toxin genes of Bacillus sphaericus produce high larvicidal activity with living cells. Western blot analysis showed that the 51-kDa and 42-kDa toxin proteins were stable in Anabaena. When a DNA fragment upstream of the 51-kDa protein gene was deleted, the toxicity was reduced by over a hundred-fold, whereas deletions at the coding regions showed that the cooperation of the two proteins expressed in Anabaena is essential for the larvicidal activity. Outdoor tests showed that the genetically altered Anabaena could keep containers with natural water from being inhabited by Culex larvae for over 2 months. Springer-Verlag New York Inc., 2000 Xu Xudong Shandong Institute of Parasitic Diseases, Jining, Shandong 272133, P. R. China, CN aut Yan Ge Shandong Institute of Parasitic Diseases, Jining, Shandong 272133, P. R. China, CN aut Kong Renqiu Shandong Institute of Parasitic Diseases, Jining, Shandong 272133, P. R. China, CN aut Liu Xiangping Shandong Institute of Parasitic Diseases, Jining, Shandong 272133, P. R. China, CN aut Yu Lihong Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan, Hubei, 430072, P. R. China, CN aut https://doi.org/10.1007/s002840010148 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010148 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Xudong Shandong Institute of Parasitic Diseases, Jining, Shandong 272133, P. R. China, CN aut NATIONALLICENCE 700 1- Yan Ge Shandong Institute of Parasitic Diseases, Jining, Shandong 272133, P. R. China, CN aut NATIONALLICENCE 700 1- Kong Renqiu Shandong Institute of Parasitic Diseases, Jining, Shandong 272133, P. R. China, CN aut NATIONALLICENCE 700 1- Liu Xiangping Shandong Institute of Parasitic Diseases, Jining, Shandong 272133, P. R. China, CN aut NATIONALLICENCE 700 1- Yu Lihong Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan, Hubei, 430072, P. R. China, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer