caa a22 4500 475746929 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s002840010150 doi (NATIONALLICENCE)springer-10.1007/s002840010150 Potassium and Sodium Fluxes in the PhytopathogenicFungus Fusarium oxysporum var. lini [Elektronische Daten] [Francisco Cabello-Hurtado, Gonzalo J. Blasco, José Ramos] Rubidium uptake in potassium-starved cells followed biphasic kinetics in the micromolar and millimolar range and was independent of the temperature. In contrast, Rb+ uptake in normal-K+ cells followed a monophasic kinetics in the millimolar range and increased at temperatures higher than 30°C. Differences in the K m values and in the Arrhenius plots of Rb+ uptake suggest different uptake systems in K+-starved and in normal-K+ cells. In addition, the substantial inhibition of Rb+ uptake caused by carbonyl cyanide-m-chlorophenyl hydrazone indicates that these systems are strongly dependent on membrane voltage. Lithium (sodium) tolerance, influx, and efflux were separately studied. F. oxysporum was shown to be very tolerant to sodium, while lithium caused a specific toxic effect. Li+ uptake in K+-starved cells exhibits a monophasic kinetics with low affinity. Li+ efflux was not affected by external pH or addition of potassium to the medium, suggesting that a Na+/cation antiporter is not involved in this process. Springer-Verlag New York Inc., 2000 Cabello-Hurtado Francisco Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14071-Córdoba, Spain, ES aut Blasco Gonzalo J. Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14071-Córdoba, Spain, ES aut Ramos José Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14071-Córdoba, Spain, ES aut https://doi.org/10.1007/s002840010150 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010150 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cabello-Hurtado Francisco Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14071-Córdoba, Spain, ES aut NATIONALLICENCE 700 1- Blasco Gonzalo J. Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14071-Córdoba, Spain, ES aut NATIONALLICENCE 700 1- Ramos José Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14071-Córdoba, Spain, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer