caa a22 4500 475746953 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s002840010144 doi (NATIONALLICENCE)springer-10.1007/s002840010144 Temperature Elevation Regulates Iron Protoporphyrin IX and Hemoglobin Binding by Porphyromonas gingivalis [Elektronische Daten] [John W. Smalley, Andrew J. Birss, Rimondia Percival, Philip D. Marsh] Porphyromonas gingivalis, an obligate anerobe with a growth requirement for iron protoporphyrin IX (FePPIX), is exposed to increased temperatures in the inflamed periodontal pocket. In this study, P. gingivalis was grown in a chemostat at 37°C (control), 39°C, and 41°C, and examined for hemagglutinating (HA) activity, hemoglobin binding and degrading activity, and iron protoporphyrin IX binding. HA activity decreased in cells as the growth temperature increased. Binding of μ-oxo bishaem (dimeric haem), and Fe(II)- and Fe(III)-monomeric forms was increased in 39°C-grown cells but decreased in 41°C-grown cells compared with controls. Cellular hemoglobin binding and degradation decreased with increased growth temperature. The decrease in cellular hemagglutination and hemoglobin degradation occurring with increased growth temperature would limit the potential overproduction of toxic monomeric haem molecules. The increased binding of μ-oxo bishaem and monomeric forms of FePPIX at 39°C may reflect a defense strategy against reactive oxidants and a mechanism of dampening down the inflammatory response to maintain an ecological balance. Springer-Verlag New York Inc., 2000 Smalley John W. Unit of Oral Biology, Department of Clinical Dental Sciences, The Edwards Building, The University of Liverpool, Liverpool L69 3GN, UK, GB aut Birss Andrew J. Unit of Oral Biology, Department of Clinical Dental Sciences, The Edwards Building, The University of Liverpool, Liverpool L69 3GN, UK, GB aut Percival Rimondia Unit of Oral Microbiology, Division of Oral Biology, Leeds Dental Institute, The University of Leeds, Leeds LS2 9LU, UK, GB aut Marsh Philip D. Unit of Oral Microbiology, Division of Oral Biology, Leeds Dental Institute, The University of Leeds, Leeds LS2 9LU, UK, GB aut https://doi.org/10.1007/s002840010144 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010144 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smalley John W. Unit of Oral Biology, Department of Clinical Dental Sciences, The Edwards Building, The University of Liverpool, Liverpool L69 3GN, UK, GB aut NATIONALLICENCE 700 1- Birss Andrew J. Unit of Oral Biology, Department of Clinical Dental Sciences, The Edwards Building, The University of Liverpool, Liverpool L69 3GN, UK, GB aut NATIONALLICENCE 700 1- Percival Rimondia Unit of Oral Microbiology, Division of Oral Biology, Leeds Dental Institute, The University of Leeds, Leeds LS2 9LU, UK, GB aut NATIONALLICENCE 700 1- Marsh Philip D. Unit of Oral Microbiology, Division of Oral Biology, Leeds Dental Institute, The University of Leeds, Leeds LS2 9LU, UK, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer