caa a22 4500 475747003 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s002840010141 doi (NATIONALLICENCE)springer-10.1007/s002840010141 Cloning, Sequencing, and Function of sanF [Elektronische Daten] A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes [Wei Chen, Hongmei Zeng, Huarong Tan] A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis. Springer-Verlag New York Inc., 2000 Chen Wei Institute of Microbiology, Chinese Academy of Sciences, P.O. Box 2714, Beijing 100080, P. R. China, CN aut Zeng Hongmei Institute of Microbiology, Chinese Academy of Sciences, P.O. Box 2714, Beijing 100080, P. R. China, CN aut Tan Huarong Institute of Microbiology, Chinese Academy of Sciences, P.O. Box 2714, Beijing 100080, P. R. China, CN aut https://doi.org/10.1007/s002840010141 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010141 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Wei Institute of Microbiology, Chinese Academy of Sciences, P.O. Box 2714, Beijing 100080, P. R. China, CN aut NATIONALLICENCE 700 1- Zeng Hongmei Institute of Microbiology, Chinese Academy of Sciences, P.O. Box 2714, Beijing 100080, P. R. China, CN aut NATIONALLICENCE 700 1- Tan Huarong Institute of Microbiology, Chinese Academy of Sciences, P.O. Box 2714, Beijing 100080, P. R. China, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer