caa a22 4500 47574702X CHVBK 20180406123516.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/PL00021057 doi (NATIONALLICENCE)springer-10.1007/PL00021057 Occurrence of Multiple Ribonucleotide Reductase Classes in γ- Proteobacteria Species [Elektronische Daten] [Eduard Torrents, Albert Jordan, Margareta Karlsson, Isidre Gibert] Ribonucleotide reductase (RNR) is central to de novo synthesis of deoxyribonucleotides and essential for all living cells. Three classes have been described; class I is oxygen dependent and represented by two subclasses, Ia (NrdAB) and Ib (NrdEF); class II (NrdJ) is indifferent to oxygen; and class III (NrdDG) is oxygen sensitive. More than one class can be found in an organism, reflecting the oxygen status of its environment. We have investigated, by using PCR and Southern blot, the occurrence of the different classes among species of the γ-Proteobacteria. Class III are present in all species tested, but the presence of the other classes varies. Some species contain one unique additional enzyme, class Ia, Ib, or II, whereas others contain two additional enzymes, class Ia and Ib, or class Ia and II. Springer-Verlag New York Inc., 2000 Torrents Eduard Department de Genètica i de Microbiologia and Institut de Biologia Fonamental, Bacterial Molecular Genetics group, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain, ES aut Jordan Albert Department de Genètica i de Microbiologia and Institut de Biologia Fonamental, Bacterial Molecular Genetics group, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain, ES aut Karlsson Margareta IT-Universitetet/KTH-Kista, Kista-förvaltningen, Electrum 214, 164 40 Kista, Sweden, SE aut Gibert Isidre Department de Genètica i de Microbiologia and Institut de Biologia Fonamental, Bacterial Molecular Genetics group, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain, ES aut https://doi.org/10.1007/PL00021057 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00021057 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Torrents Eduard Department de Genètica i de Microbiologia and Institut de Biologia Fonamental, Bacterial Molecular Genetics group, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain, ES aut NATIONALLICENCE 700 1- Jordan Albert Department de Genètica i de Microbiologia and Institut de Biologia Fonamental, Bacterial Molecular Genetics group, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain, ES aut NATIONALLICENCE 700 1- Karlsson Margareta IT-Universitetet/KTH-Kista, Kista-förvaltningen, Electrum 214, 164 40 Kista, Sweden, SE aut NATIONALLICENCE 700 1- Gibert Isidre Department de Genètica i de Microbiologia and Institut de Biologia Fonamental, Bacterial Molecular Genetics group, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer