caa a22 4500 475747046 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s002840010101 doi (NATIONALLICENCE)springer-10.1007/s002840010101 An ACC Deaminase Minus Mutant of Enterobacter cloacae UW4No Longer Promotes Root Elongation [Elektronische Daten] [Jiping Li, Daniel H. Ovakim, Trevor C. Charles, Bernard R. Glick] The ACC deaminase gene (acdS) from Enterobacter cloacae UW4 was replaced by homologous recombination with the acdS gene with a tetracycline resistance gene inserted within the coding region. Upon characterization of this AcdS minus mutant, it was determined that both ACC deaminase activity and the ability to promote the elongation of canola roots under gnotobiotic conditions were greatly diminished. This result is consistent with a previously postulated model that suggests that a major mechanism utilized by plant growth-promoting bacteria involves the lowering of plant ethylene levels, and hence ethylene inhibition of root elongation, by bacterial ACC deaminase. Springer-Verlag New York Inc., 2000 Li Jiping Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut Ovakim Daniel H. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut Charles Trevor C. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut Glick Bernard R. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut https://doi.org/10.1007/s002840010101 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010101 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Jiping Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut NATIONALLICENCE 700 1- Ovakim Daniel H. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut NATIONALLICENCE 700 1- Charles Trevor C. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut NATIONALLICENCE 700 1- Glick Bernard R. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer