caa a22 4500 475747097 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s002840010103 doi (NATIONALLICENCE)springer-10.1007/s002840010103 High-Resolution Physical Map of the pSymb Megaplasmid and Comparison of the Three Replicons of Sinorhizobium meliloti Strain 1021 [Elektronische Daten] [Frédérique Barloy-Hubler, Delphine Capela, Jacques Batut, Francis Galibert] A high-resolution physical map of the larger megaplasmid (pSymb) of Sinorhizobium meliloti strain 1021 has been constructed by using BAC libraries and an original two-step PCR screening method. This method, previously used to map both the chromosome and the smaller megaplasmid (pSyma), allowed us to position over the genome a total of 842 markers with an average density of one marker every 8.3 kb. In addition, we used BLASTX and PRODOM analysis to predict a function for a number of STSs. This work led to the discovery of several interesting loci and to a comparison of the genetic information carried by each replicon. The two main results emerging from this study are (i) a biased distribution of housekeeping genes, mainly detected on chromosome, and (ii) the presence of an unexpected number of transporters, mainly belonging to the ABC superfamily. These are broadly distributed across the whole genome, but particularly found on pSymb. Springer-Verlag New York Inc., 2000 Barloy-Hubler Frédérique Laboratoire Recombinaisons Génétiques CNRS-UPR41 CNRS, 2 Avenue du Pr Léon Bernard, 35043 Rennes cedex, France, FR aut Capela Delphine Laboratoire Recombinaisons Génétiques CNRS-UPR41 CNRS, 2 Avenue du Pr Léon Bernard, 35043 Rennes cedex, France, FR aut Batut Jacques Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes UMR215 INRA-CNRS, Chemin de Borde Rouge, BP27, 31326 Castanet-Tolosan cedex, France, FR aut Galibert Francis Laboratoire Recombinaisons Génétiques CNRS-UPR41 CNRS, 2 Avenue du Pr Léon Bernard, 35043 Rennes cedex, France, FR aut https://doi.org/10.1007/s002840010103 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010103 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barloy-Hubler Frédérique Laboratoire Recombinaisons Génétiques CNRS-UPR41 CNRS, 2 Avenue du Pr Léon Bernard, 35043 Rennes cedex, France, FR aut NATIONALLICENCE 700 1- Capela Delphine Laboratoire Recombinaisons Génétiques CNRS-UPR41 CNRS, 2 Avenue du Pr Léon Bernard, 35043 Rennes cedex, France, FR aut NATIONALLICENCE 700 1- Batut Jacques Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes UMR215 INRA-CNRS, Chemin de Borde Rouge, BP27, 31326 Castanet-Tolosan cedex, France, FR aut NATIONALLICENCE 700 1- Galibert Francis Laboratoire Recombinaisons Génétiques CNRS-UPR41 CNRS, 2 Avenue du Pr Léon Bernard, 35043 Rennes cedex, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer