caa a22 4500 475747100 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s002840010100 doi (NATIONALLICENCE)springer-10.1007/s002840010100 wsp Gene Sequences from the Wolbachia of Filarial Nematodes [Elektronische Daten] [Chiara Bazzocchi, Wanwisa Jamnongluk, Scott L. O'Neill, Tim J.C. Anderson, Claudio Genchi, Claudio Bandi] Wolbachia endosymbiotic bacteria are widespread in arthropods and are also present in filarial nematodes. Almost all filarial species so far examined have been found to harbor these endosymbionts. The sequences of only three genes have been published for nematode Wolbachia (i.e., the genes coding for the proteins FtsZ and catalase and for 16S rRNA). Here we present the sequences of the genes coding for the Wolbachia surface protein (WSP) from the endosymbionts of eight species of filaria. Complete gene sequences were obtained from the endosymbionts of two different species, Dirofilaria immitis and Brugia malayi. These sequences allowed us to design general primers for amplification of the wsp gene from the Wolbachia of all filarial species examined. For these species, partial WSP sequences (about 600 base pairs) were obtained with these primers. Phylogenetic analysis groups these nematode wsp sequences into a coherent cluster. Within the nematode cluster, wsp-based Wolbachia phylogeny matches a previous phylogeny obtained with ftsZ gene sequences, with a good consistency of the phylogeny of hosts (nematodes) and symbionts (Wolbachia). In addition, different individuals of the same host species (Dirofilaria immitis and Wuchereria bancrofti) show identical wsp gene sequences. Springer-Verlag New York Inc., 2000 Bazzocchi Chiara Istituto di Patologia Generale Veterinaria, Via Celoria 10, 20133 Milano, Italy, IT aut Jamnongluk Wanwisa Department of Epidemiology and Public Health, Yale University, New Haven, CT 06520, USA, US aut O'Neill Scott L. Department of Epidemiology and Public Health, Yale University, New Haven, CT 06520, USA, US aut Anderson Tim J.C. Istituto di Patologia Generale Veterinaria, Via Celoria 10, 20133 Milano, Italy, IT aut Genchi Claudio Istituto di Patologia Generale Veterinaria, Via Celoria 10, 20133 Milano, Italy, IT aut Bandi Claudio Istituto di Patologia Generale Veterinaria, Via Celoria 10, 20133 Milano, Italy, IT aut https://doi.org/10.1007/s002840010100 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010100 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bazzocchi Chiara Istituto di Patologia Generale Veterinaria, Via Celoria 10, 20133 Milano, Italy, IT aut NATIONALLICENCE 700 1- Jamnongluk Wanwisa Department of Epidemiology and Public Health, Yale University, New Haven, CT 06520, USA, US aut NATIONALLICENCE 700 1- O'Neill Scott L. Department of Epidemiology and Public Health, Yale University, New Haven, CT 06520, USA, US aut NATIONALLICENCE 700 1- Anderson Tim J.C. Istituto di Patologia Generale Veterinaria, Via Celoria 10, 20133 Milano, Italy, IT aut NATIONALLICENCE 700 1- Genchi Claudio Istituto di Patologia Generale Veterinaria, Via Celoria 10, 20133 Milano, Italy, IT aut NATIONALLICENCE 700 1- Bandi Claudio Istituto di Patologia Generale Veterinaria, Via Celoria 10, 20133 Milano, Italy, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer