caa a22 4500 475747119 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s002840010104 doi (NATIONALLICENCE)springer-10.1007/s002840010104 Nitrate Reductase and Nitrous Oxide Production by Fusarium oxysporum 11dn1 Under Aerobic and Anaerobic Conditions [Elektronische Daten] [Alexander V. Kurakov, Alexander N. Nosikov, Evdokiya V. Skrynnikova, Nicolay P. L'vov] The fungus Fusarium oxysporum 11dn1 was found to be able to grow and produce nitrous oxide on nitrate-containing medium in anaerobic conditions. The rate of nitrous oxide formation was three to six orders of magnitude lower than the rates of molecular nitrogen production by common denitrifying bacteria. Acetylene and ammonia did not affect the release of nitrous oxide release. It was shown that under anaerobic conditions fast increase of nitrate reductase activity occurred, caused by the synthesis of enzyme de novo and protein dephosphorylation. Reverse transfer of the mycelium to aerobic conditions led to a decline in nitrate reductase activity and stopped nitrous oxide production. The presence of two nitrate reductases was shown, which differed in molecular mass, location, temperature optima, and activity in nitrate- and ammonium-containing media. Two enzymes represent assimilatory and dissimilatory nitrate reductases, which are active in aerobic and anaerobic conditions, respectively. Springer-Verlag New York Inc., 2000 Kurakov Alexander V. Moscow Lomonosov State University, International Biotechnological Center and Soil Biology Department, Vorob'evy gory, Moscow, 119899, Russia, RU aut Nosikov Alexander N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr.33, Moscow, 117071, Russia, RU aut Skrynnikova Evdokiya V. Moscow Lomonosov State University, International Biotechnological Center and Soil Biology Department, Vorob'evy gory, Moscow, 119899, Russia, RU aut L'vov Nicolay P. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr.33, Moscow, 117071, Russia, RU aut https://doi.org/10.1007/s002840010104 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010104 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kurakov Alexander V. Moscow Lomonosov State University, International Biotechnological Center and Soil Biology Department, Vorob'evy gory, Moscow, 119899, Russia, RU aut NATIONALLICENCE 700 1- Nosikov Alexander N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr.33, Moscow, 117071, Russia, RU aut NATIONALLICENCE 700 1- Skrynnikova Evdokiya V. Moscow Lomonosov State University, International Biotechnological Center and Soil Biology Department, Vorob'evy gory, Moscow, 119899, Russia, RU aut NATIONALLICENCE 700 1- L'vov Nicolay P. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr.33, Moscow, 117071, Russia, RU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer