caa a22 4500 475747127 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s002840010107 doi (NATIONALLICENCE)springer-10.1007/s002840010107 Adherence of Lactobacillus to Intestinal 407 Cells in Culture Correlates with Fibronectin Binding [Elektronische Daten] [Darrell R. Kapczynski, Richard J. Meinersmann, Margie D. Lee] Lactobacilli are members of the normal mucosal microflora of most animals. Many isolates of Lactobacillus spp. are adherent to epithelial cells. In this study, using Lactobacillus acidophilus and L. agilis, we detected adherence in a pattern that suggested that the bacteria were binding to extracellular matrix proteins. Fluorescent microscopy, by using anti-fibronectin antibody, demonstrated that the isolates localize in those areas where fibronectin was detected. In addition, fibronectin pretreatment of the bacterial cells decreased adherence to Intestinal 407 epithelial cell monolayers. Cellular binding to fibronectin was detected by enzyme-linked immunosorbent assay and affinity binding to radio-labeled fibronectin. Fibronectin may be one of the eukaryotic receptors mediating attachment of Lactobacillus to mucosal surfaces. Springer-Verlag New York Inc., 2000 Kapczynski Darrell R. Southeast Poultry Research, USDA/ARS, Athens, GA, USA, US aut Meinersmann Richard J. Poultry Processing and Meat Quality Research, USDA/ARS, Athens, GA, USA, US aut Lee Margie D. Department of Medical Microbiology and Parasitology, The University of Georgia, Athens, GA 30602, USA, US aut https://doi.org/10.1007/s002840010107 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010107 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kapczynski Darrell R. Southeast Poultry Research, USDA/ARS, Athens, GA, USA, US aut NATIONALLICENCE 700 1- Meinersmann Richard J. Poultry Processing and Meat Quality Research, USDA/ARS, Athens, GA, USA, US aut NATIONALLICENCE 700 1- Lee Margie D. Department of Medical Microbiology and Parasitology, The University of Georgia, Athens, GA 30602, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer