caa a22 4500 475747992 CHVBK 20180406123519.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1023/A:1006698728816 doi (NATIONALLICENCE)springer-10.1023/A:1006698728816 Distal Proctocolitis and n-3 Polyunsaturated Fatty Acids (n-3 PUFAs): The Mucosal Effect In Situ [Elektronische Daten] [Y. Almallah, Stanley Ewen, Amir El-Tahir, N. Mowat, Peter Brunt, Thomas Sinclair, Steven Heys, Oleg Eremin] It has been postulated that patients with ulcerative colitis (UC) have altered reactivity of gut-associated lymphoid tissue. In such cases there is intense infiltration of the mucosa with immune competent cells and associated tissue damage. We have shown previously that the dietary supplementation with the n-3 polyunsaturated fatty acids (n-3 PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) results in significant systemic immune suppression. The aim of this study, therefore, was to evaluate the in situ effect of n-3 PUFAs on distal proctocolitis. Each patient received either fish oil extract (EPA 3.2 g, DHA 2.4 g) (n = 9) or sunflower oil (n = 9) daily in a double blind manner for six months. Monthly assessment included: (1) disease activity using clinical, sigmoidoscopic, and histological scores and (2) immunohistochemical analysis (immunoglobulins, CD profiles) of rectal biopsy specimens (before and after six months supplementation) using monoclonal antibodies and quantitative computer-assisted video image analysis. Prior to receiving supplementation, patients with proctocolitis (n = 18) showed significantly higher numbers of cells expressing CD3 (pan T cells) and HLA-DR and IgM containing cells compared with non-colitic controls (n = 8). Six months supplementation with n-3 PUFAs resulted in significant reduction in the number of cells expressing CD3 and HLA and the percentage of cells containing IgM. There was no significant change in the CD20 nor the percentage of IgG or IgA containing cells in either group of patients with procto-colitis. In patients receiving n-3 PUFA supplementation, there was improvement in the disease activity and histological scores, compared with pretreatment evaluation. This study has demonstrated both evidence of suppression of in situ immune reactivity and concurrent reduction in disease activity in patients with proctocolitis receiving n-3 PUFA supplementation. This may have important implication for therapy in patients with ulcerative colitis. Plenum Publishing Corporation, 2000 n-3 polyunsaturated fatty acids nationallicence procto-colitis nationallicence imunohistochemistry nationallicence Almallah Y. Department of Surgery, University of Aberdeen, Aberdeen, United Kingdom aut Ewen Stanley Department of Pathology, Aberdeen, United Kingdom aut El-Tahir Amir Department of Surgery, University of Aberdeen, Aberdeen, United Kingdom aut Mowat N. Gastrointestinal Unit, Aberdeen Royal Infirmary NHS Trust, Aberdeen, United Kingdom aut Brunt Peter Gastrointestinal Unit, Aberdeen Royal Infirmary NHS Trust, Aberdeen, United Kingdom aut Sinclair Thomas Gastrointestinal Unit, Aberdeen Royal Infirmary NHS Trust, Aberdeen, United Kingdom aut Heys Steven Department of Surgery, University of Aberdeen, Aberdeen, United Kingdom aut Eremin Oleg Department of Surgery, University of Aberdeen, Aberdeen, United Kingdom aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/1(2000-01-01), 68-76 0271-9142 20:1<68 2000 20 10875 https://doi.org/10.1023/A:1006698728816 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1006698728816 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Almallah Y. Department of Surgery, University of Aberdeen, Aberdeen, United Kingdom aut NATIONALLICENCE 700 1- Ewen Stanley Department of Pathology, Aberdeen, United Kingdom aut NATIONALLICENCE 700 1- El-Tahir Amir Department of Surgery, University of Aberdeen, Aberdeen, United Kingdom aut NATIONALLICENCE 700 1- Mowat N. Gastrointestinal Unit, Aberdeen Royal Infirmary NHS Trust, Aberdeen, United Kingdom aut NATIONALLICENCE 700 1- Brunt Peter Gastrointestinal Unit, Aberdeen Royal Infirmary NHS Trust, Aberdeen, United Kingdom aut NATIONALLICENCE 700 1- Sinclair Thomas Gastrointestinal Unit, Aberdeen Royal Infirmary NHS Trust, Aberdeen, United Kingdom aut NATIONALLICENCE 700 1- Heys Steven Department of Surgery, University of Aberdeen, Aberdeen, United Kingdom aut NATIONALLICENCE 700 1- Eremin Oleg Department of Surgery, University of Aberdeen, Aberdeen, United Kingdom aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/1(2000-01-01), 68-76 0271-9142 20:1<68 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer