caa a22 4500 475748077 CHVBK 20180406123519.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1023/A:1006694627907 doi (NATIONALLICENCE)springer-10.1023/A:1006694627907 Relationship Between Interferon-γ, Interleukin-10, and Interleukin-12 Production in Chronic Hepatitis C and In Vitro Effects of Interferon-α [Elektronische Daten] [Giuseppina Piazzolla, Cosimo Tortorella, Oronzo Schiraldi, Salvatore Antonaci] In the current study, increased interferon (IFN)-γ, interleukin (IL)-10, and IL-12 p40 serum levels were observed in patients with chronic hepatitis C (CHC) compared to controls. Patients also displayed an increased spontaneous IFN-γ release but a deficient peripheral blood mononuclear cells (PBMC) IFN-γ production following stimulation with Staphylococcus aureus Cowan I strain (SAC). No difference was found with reference to spontaneous or phytohaemagglutinin (PHA)-induced IL-10 release between patients and controls, whereas a higher IL-12 p70 and IL-12 p40 secretion triggered by SAC was observed in patients. Moreover, IL-12 p40/p70 ratio following SAC stimulation was higher in patients compared to controls and a negative correlation was found between this ratio and IFN-γ amounts. Recombinant IL-12 (rIL-12) as well as neutralizing anti-IL-10 monoclonal antibodies (mAbs) were able to restore the compromised IFN-γ production. Of note, anti-IL-10 supplementation induced a lower IL-12 p40/p70 ratio in HCV subjects as compared to controls. Finally, IFN-α upregulated in vitro IFN-γ, IL-10, and IL-12 p70 release but not IL-12 p40 secretion, this giving rise to a normalization of IL-12 p40/p70 ratio. The data suggest the occurrence of an enhanced responsiveness to IL-10 modulating effects, likely mediated by an imbalance of IL-12 p40/p70 ratio, in chronic HCV infection. Cytokine balance restoration might thus contribute to achieve therapeutical results in chronic hepatitis C. Plenum Publishing Corporation, 2000 Chronic hepatitis C nationallicence IFN-γ nationallicence IL-10 nationallicence IL-12 p70, IL-12 p40 nationallicence Piazzolla Giuseppina Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical School, Bari, Italy aut Tortorella Cosimo Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical School, Bari, Italy aut Schiraldi Oronzo Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical School, Bari, Italy aut Antonaci Salvatore Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical School, Bari, Italy aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/1(2000-01-01), 54-61 0271-9142 20:1<54 2000 20 10875 https://doi.org/10.1023/A:1006694627907 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1006694627907 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Piazzolla Giuseppina Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical School, Bari, Italy aut NATIONALLICENCE 700 1- Tortorella Cosimo Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical School, Bari, Italy aut NATIONALLICENCE 700 1- Schiraldi Oronzo Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical School, Bari, Italy aut NATIONALLICENCE 700 1- Antonaci Salvatore Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical School, Bari, Italy aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/1(2000-01-01), 54-61 0271-9142 20:1<54 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer