caa a22 4500 475748115 CHVBK 20180406123519.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1023/A:1026411916855 doi (NATIONALLICENCE)springer-10.1023/A:1026411916855 Cytokine Analysis at the Single Cell Level and Lymphoproliferative Responses to Mycobacterial Antigens in HIV-1 Patients with Successful Virologic Response to Potent Antiretrovirals [Elektronische Daten] [Corinne Amiel, Jean-Pierre Kusnierz, Yves Mouton, Graham Rook, John Stanford, Mahavir Singh, Andre Capron, George Bahr] Immunologic parameters, known to be grossly abnormal in HIV-1-infected subjects, were analyzed in 22 patients with sustained viral load suppression (<200 copies/ml) following long-term highly active antiretroviral therapy (HAART). Responses were compared with those from 18 HIV-seronegative healthy controls. Persistent phenotypic alterations in patients' blood mononuclear cells were minimal, though the percentages of lymphocytes that could be activated to produce interleukin-2 (IL-2) remained severely depressed. Using lymphoproliferative assays, a striking deficit in the capacity of patients to respond to the common mycobacterial antigens and particularly to recombinant heat-shock proteins paralleled the absence of responses to virus p24 antigen. In view of the important immunoregulatory role of stress proteins, these findings reveal profound functional deficiencies and persistent immune dysregulation in HIV-1 patients, despite successful HAART and a considerable recovery of CD4+ lymphocyte numbers. Rational immunotherapeutic approaches should be aimed to correct the characterized immune abnormalities. Plenum Publishing Corporation, 2000 HIV-1 nationallicence immune reconstitution nationallicence heat-shock proteins nationallicence lymphoproliferation nationallicence intracellular cytokines nationallicence Amiel Corinne Laboratory of Molecular Immunology of Infections and Inflammation, Institut Pasteur de Lille, France aut Kusnierz Jean-Pierre Laboratory of Molecular Immunology of Infections and Inflammation, Institut Pasteur de Lille, France aut Mouton Yves Department of Infectious Diseases, Dron Hospital, Tourcoing, France aut Rook Graham Royal Free and University College Medical School, London, England aut Stanford John Royal Free and University College Medical School, London, England aut Singh Mahavir German National Research Center for Biotechnology, Braunschweig, Germany aut Capron Andre Laboratory of Molecular Immunology of Infections and Inflammation, Institut Pasteur de Lille, France aut Bahr George Laboratory of Molecular Immunology of Infections and Inflammation, Institut Pasteur de Lille, France aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/6(2000-11-01), 458-465 0271-9142 20:6<458 2000 20 10875 https://doi.org/10.1023/A:1026411916855 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1026411916855 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Amiel Corinne Laboratory of Molecular Immunology of Infections and Inflammation, Institut Pasteur de Lille, France aut NATIONALLICENCE 700 1- Kusnierz Jean-Pierre Laboratory of Molecular Immunology of Infections and Inflammation, Institut Pasteur de Lille, France aut NATIONALLICENCE 700 1- Mouton Yves Department of Infectious Diseases, Dron Hospital, Tourcoing, France aut NATIONALLICENCE 700 1- Rook Graham Royal Free and University College Medical School, London, England aut NATIONALLICENCE 700 1- Stanford John Royal Free and University College Medical School, London, England aut NATIONALLICENCE 700 1- Singh Mahavir German National Research Center for Biotechnology, Braunschweig, Germany aut NATIONALLICENCE 700 1- Capron Andre Laboratory of Molecular Immunology of Infections and Inflammation, Institut Pasteur de Lille, France aut NATIONALLICENCE 700 1- Bahr George Laboratory of Molecular Immunology of Infections and Inflammation, Institut Pasteur de Lille, France aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/6(2000-11-01), 458-465 0271-9142 20:6<458 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer