caa a22 4500 475748158 CHVBK 20180406123519.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1023/A:1026407815946 doi (NATIONALLICENCE)springer-10.1023/A:1026407815946 Low In Vitro Production of Interferon-γ and Tumor Necrosis Factor-α in HIV-Seronegative Patients with Pulmonary Disease Caused by Nontuberculous Mycobacteria [Elektronische Daten] [Ulf Greinert, Max Schlaak, Sabine Rũsch-Gerdes, Hans-Dieter Flad, Martin Ernst] We studied 32 HIV-seronegative patients with pulmonary disease caused by nontuberculous mycobacteria (NTM). Immunologic studies included lymphocyte subset analysis by flow cytometry, measurement of interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) production followingin vitro stimulation of diluted whole blood (DWB) and peripheral blood mononuclear cells (PBMC) by phytohemagglutinin (PHA), anti-CD3 as well as purified protein derivative of tuberculin (PPD), and in four cases with different amounts of the very mycobacterium, which caused disease in these patients. Data were compared to those of 30 HIV-seronegative patients with disease byMycobacterium tuberculosis (MTb). Following α-CD3-stimulation of PBMC, NTM patients showed lower IFN-γ(P < 0.00005) and lower TNF-α(P < 0.02). For a subgroup of tuberculin skin test-positive NTM patients we found significantly lower PPD-induced IFN-γ releases in cultured DWB(P < 0.0002) and PBMC(P < 0.0004) compared to MTb patients. Data for PPD-induced TNF-α release for this subgroup were also significant(P < 0.001 andP < 0.05, respectively). The four NTM patients with poor PPD-induced IFN-γ response hardly showed increased cytokine production on stimulation with their specific mycobacterium. The lower production capacity of IFN-γ and TNF-α of NTM patients compared to the MTb patients points to an immunologic imbalance forming the basis for their increased susceptibility to pulmonary infections by nontuberculous mycobacteria. Plenum Publishing Corporation, 2000 Nontuberculous mycobacteria nationallicence tuberculosis nationallicence in vitro immune response nationallicence interferon-γ nationallicence tumor necrosis factor-α nationallicence Greinert Ulf Medizinische Klinik, Forschungszentrum Borstel, Borstel, Germany aut Schlaak Max Medizinische Klinik, Forschungszentrum Borstel, Borstel, Germany aut Rũsch-Gerdes Sabine Nationales Referenzzentrum f¨ur Mykobakterien, Forschungszentrum Borstel, Borstel, Germany aut Flad Hans-Dieter Abteilung f¨ur Immunologie und Zellbiologie, Forschungszentrum Borstel, Borstel, Germany aut Ernst Martin Abteilung f¨ur Immunologie und Zellbiologie, Forschungszentrum Borstel, Borstel, Germany aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/6(2000-11-01), 445-452 0271-9142 20:6<445 2000 20 10875 https://doi.org/10.1023/A:1026407815946 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1026407815946 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Greinert Ulf Medizinische Klinik, Forschungszentrum Borstel, Borstel, Germany aut NATIONALLICENCE 700 1- Schlaak Max Medizinische Klinik, Forschungszentrum Borstel, Borstel, Germany aut NATIONALLICENCE 700 1- Rũsch-Gerdes Sabine Nationales Referenzzentrum f¨ur Mykobakterien, Forschungszentrum Borstel, Borstel, Germany aut NATIONALLICENCE 700 1- Flad Hans-Dieter Abteilung f¨ur Immunologie und Zellbiologie, Forschungszentrum Borstel, Borstel, Germany aut NATIONALLICENCE 700 1- Ernst Martin Abteilung f¨ur Immunologie und Zellbiologie, Forschungszentrum Borstel, Borstel, Germany aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/6(2000-11-01), 445-452 0271-9142 20:6<445 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer