caa a22 4500 475748212 CHVBK 20180406123519.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1023/A:1026455631876 doi (NATIONALLICENCE)springer-10.1023/A:1026455631876 Exposure to Topical Bovine Thrombin During Surgery Elicits a Response Against the Xenogeneic Carbohydrate Galactose α1-3Galactose [Elektronische Daten] [Jonathan Schoenecker, Rachel Hauck, Meg Mercer, William Parker, Jeffrey Lawson] Exposure of humans to topical bovine thrombin has been associated with development of antibodies against bovine and human coagulation factors and blood coagulation abnormalities. However, the nature of this humoral response is unknown. In this study, numerous glycoproteins in the topical bovine thrombin were found to contain the Galα1-3Gal epitope, which is known to be highly immunogenic. More importantly, Galα1-3Gal is recognized by natural antibodies that are found in all normal individuals and are known to effectively mediate complement activation and subsequent destruction of xenogeneic tissues. Thus, primary exposure of normal individuals to topical bovine thrombin is expected to result in an immediate immune reaction against that reagent. Further, following exposure to topical bovine thrombin, the levels of anti-Galα1-3Gal IgG rose to levels tenfold greater than the average level of natural anti-Galα1-3Gal IgG in naive individuals. Thus, Galα1-3Gal in topical bovine thrombin accounts for, at least in part, the highly immunogenic nature of this reagent. Plenum Publishing Corporation, 2000 Galactoseα1-3Galactose nationallicence topical bovine thrombin nationallicence natural antibodies nationallicence surgery nationallicence species specificity nationallicence autoimmunity nationallicence Schoenecker Jonathan Department of Surgery, Duke University Medical Center, 27710, Durham, North Carolina aut Hauck Rachel Department of Surgery, Duke University Medical Center, 27710, Durham, North Carolina aut Mercer Meg Department of Surgery, Duke University Medical Center, 27710, Durham, North Carolina aut Parker William Department of Surgery, Duke University Medical Center, 27710, Durham, North Carolina aut Lawson Jeffrey Department of Surgery, Duke University Medical Center, 27710, Durham, North Carolina aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/6(2000-11-01), 434-444 0271-9142 20:6<434 2000 20 10875 https://doi.org/10.1023/A:1026455631876 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1026455631876 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schoenecker Jonathan Department of Surgery, Duke University Medical Center, 27710, Durham, North Carolina aut NATIONALLICENCE 700 1- Hauck Rachel Department of Surgery, Duke University Medical Center, 27710, Durham, North Carolina aut NATIONALLICENCE 700 1- Mercer Meg Department of Surgery, Duke University Medical Center, 27710, Durham, North Carolina aut NATIONALLICENCE 700 1- Parker William Department of Surgery, Duke University Medical Center, 27710, Durham, North Carolina aut NATIONALLICENCE 700 1- Lawson Jeffrey Department of Surgery, Duke University Medical Center, 27710, Durham, North Carolina aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/6(2000-11-01), 434-444 0271-9142 20:6<434 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer