caa a22 4500 475748220 CHVBK 20180406123519.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1023/A:1026478914129 doi (NATIONALLICENCE)springer-10.1023/A:1026478914129 Complexes of Serum Amyloid P Component and DNA in Serum from Healthy Individuals and Systemic Lupus Erythematosus Patients [Elektronische Daten] [Inge Sørensen, Ellen Nielsen, Lise Schrøder, Anne Voss, Laura Horváth, Sven-Erik Svehag] Serum amyloid P component (SAP) bindsin vitro to DNA; based on findings in SAP-deficient mice it was proposed that SAP's role is to handle chromatin and DNA, thereby preventing formation of anti-DNA antibodies. For the first time we have shown the presence of Ca2+-dependent SAP-DNA complexes, measured by ELISA, in sera from both healthy volunteers and systemic lupus erythematosus patients (SLE). The concentration of SAP-DNA complexes in SLE sera was significantly lower than in normal sera and particularly low in sera from patients with anti-DNA titers exceeding 50. The complexes were dissociated by the SAP ligand heparin and were not demonstrable in EDTA plasma. Normal sera showed similar capacity to form SAP-DNA complexes with both thymus andEscherichia coli DNA, whereas significantly lower amounts of complexes, in particular withE. coli DNA, were formed in SLE sera. SLE patients with moderate to high anti-DNA titers showed a significant negative correlation between serum SAP's binding ofE. coli DNA and the anti-DNA titer. Plenum Publishing Corporation, 2000 Serum amyloid P component nationallicence vertebrate and bacterial DNA nationallicence systemic lupus erythematosus nationallicence Sørensen Inge Department of Internal Medicine C, Section of Rheumatology, Odense University Hospital, Odense, Denmark aut Nielsen Ellen Department of Anatomy and Neurobiology, SDU—Odense University, Odense, Denmark aut Schrøder Lise Department of Immunology and Microbiology, SDU—Odense University, Odense, Denmark aut Voss Anne Department of Internal Medicine C, Section of Rheumatology, Odense University Hospital, Odense, Denmark aut Horváth Laura Third Department of Medicine, Semmelweis Medical University, Budapest, Hungary aut Svehag Sven-Erik Department of Immunology and Microbiology, SDU—Odense University, Odense, Denmark aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/6(2000-11-01), 408-415 0271-9142 20:6<408 2000 20 10875 https://doi.org/10.1023/A:1026478914129 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1026478914129 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sørensen Inge Department of Internal Medicine C, Section of Rheumatology, Odense University Hospital, Odense, Denmark aut NATIONALLICENCE 700 1- Nielsen Ellen Department of Anatomy and Neurobiology, SDU—Odense University, Odense, Denmark aut NATIONALLICENCE 700 1- Schrøder Lise Department of Immunology and Microbiology, SDU—Odense University, Odense, Denmark aut NATIONALLICENCE 700 1- Voss Anne Department of Internal Medicine C, Section of Rheumatology, Odense University Hospital, Odense, Denmark aut NATIONALLICENCE 700 1- Horváth Laura Third Department of Medicine, Semmelweis Medical University, Budapest, Hungary aut NATIONALLICENCE 700 1- Svehag Sven-Erik Department of Immunology and Microbiology, SDU—Odense University, Odense, Denmark aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/6(2000-11-01), 408-415 0271-9142 20:6<408 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer