caa a22 4500 475748271 CHVBK 20180406123519.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1023/A:1006685413157 doi (NATIONALLICENCE)springer-10.1023/A:1006685413157 Immune Response of SLE Patients to Peptides Based on the Complementarity Determining Regions of a Pathogenic Anti-DNA Monoclonal Antibody [Elektronische Daten] [Molly Dayan, Raphael Segal, Zev Sthoeger, Ari Waisman, Na'ama Brosh, Ori Elkayam, Eran Eilat, Mati Fridkin, Edna Mozes] We have examined the humoral and cellular responses of SLE patients to peptides based on the complementarity-determining regions (CDR) of a monoclonal anti-DNA antibody with a major idiotype-16/6 Id, in comparison to their responses to the whole 16/6 Id-bearing antibody. Sera of 63% of the SLE patients had antibodies that bound the 16/6 Id, 80% had antibodies to one of the CDR-based peptides, and 40% of the patients reacted with both CDRs. Sera of only a few controls reacted with either the 16/6 Id (6%) or the CDR based peptides (4%) (P < 0.01). Peripheral blood lymphocytes (PBL) of 39% of the patients proliferated in response to the 16/6 Id or to one of the CDR-based peptides (37%), while in the control group the proliferation rates were 66% to the 16/6 Id and 59% to one of the CDR-based peptides (P < 0.05). The correlation between (both) the humoral and cellular immune responses to the CDR-based peptides and to the 16/6 Id suggests the relevance of these peptides to the 16/6 Id and provides additional information on the pathogenic moiety of the latter antibody. Plenum Publishing Corporation, 2000 SLE nationallicence CDR-based peptides nationallicence T-cell proliferation nationallicence antigen presentation nationallicence Dayan Molly Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut Segal Raphael Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut Sthoeger Zev aut Waisman Ari Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut Brosh Na'ama Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut Elkayam Ori Department of Internal Medicine B, Kaplan Hospital, 76100, Rehovot, Israel aut Eilat Eran Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut Fridkin Mati Department of Organic Chemistry, The Weizmann Institute of Science, 76100, Rehovot, Israel aut Mozes Edna Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/3(2000-05-01), 187-194 0271-9142 20:3<187 2000 20 10875 https://doi.org/10.1023/A:1006685413157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1006685413157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dayan Molly Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Segal Raphael Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Sthoeger Zev aut NATIONALLICENCE 700 1- Waisman Ari Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Brosh Na'ama Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Elkayam Ori Department of Internal Medicine B, Kaplan Hospital, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Eilat Eran Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Fridkin Mati Department of Organic Chemistry, The Weizmann Institute of Science, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Mozes Edna Department of Immunology, The Weizmann Institute of Science, 76100, Rehovot, Israel aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/3(2000-05-01), 187-194 0271-9142 20:3<187 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer