caa a22 4500 47574828X CHVBK 20180406123519.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1023/A:1006689514066 doi (NATIONALLICENCE)springer-10.1023/A:1006689514066 Antigen-Specific Regulation of CD1 Expression in Humans [Elektronische Daten] [Marina Ulanova, Andrej Tarkowski, Steven Porcelli, Lars Hanson] During the last decade the CD1 family of cell surface glycoproteins has been implicated in the presentation of nonpeptide antigens in man. Recent findings by our group indicate that CD1 molecules also can be involved in the presentation of certain bacterial proteins. However, CD1a, b, and c (group 1 CD1 molecules) are not present at significant levels on circulating monocytes unless their expression is induced by cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF). In this study we investigated the cell surface expression of CD1 molecules following the antigenic stimulation in vivo via immunization of healthy volunteers with tetanus toxoid vaccine and in vitro cell cultures using the same antigen. Both the in vivo and in vitro studies demonstrated clear up-regulation of the surface expression of CD1a, b, and c on monocytes as a result of antigenic stimulation with tetanus toxoid, supporting the idea that CD1 molecules participate in the presentation of this protein antigen in man. In vitro, antigen-triggered expression of these molecules was mediated by GM-CSF, since neutralization of this cytokine with specific antibody totally abrogated CD1a, b, and c expression. In contrast to the group 1 CD1 molecules, CD1d was found to be constitutively expressed on the majority of circulating monocytes and B lymphocytes prior to immunization. There was no effect of antigenic stimulation with tetanus toxoid on the cell surface expression of CD1d, suggesting major differences in regulation of the expression and function of the different CD1 molecules in humans. Altogether our results point to antigen-driven up-regulation of CD1a, b, and c expression on human monocytes that is mediated by GM-CSF and no effect on CD1d expression. Plenum Publishing Corporation, 2000 CD1 nationallicence monocytes nationallicence tetanus toxoid nationallicence GM-CSF nationallicence Ulanova Marina Department of Clinical Immunology, University of G¨oteborg, S-41346, G¨oteborg, Sweden aut Tarkowski Andrej Department of Rheumatology, University of G¨oteborg, S-41346, G¨oteborg, Sweden aut Porcelli Steven Lymphocyte Biology Section, Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, 02115, Boston, Massachusetts aut Hanson Lars Department of Clinical Immunology, University of G¨oteborg, S-41346, G¨oteborg, Sweden aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/3(2000-05-01), 203-211 0271-9142 20:3<203 2000 20 10875 https://doi.org/10.1023/A:1006689514066 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1006689514066 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ulanova Marina Department of Clinical Immunology, University of G¨oteborg, S-41346, G¨oteborg, Sweden aut NATIONALLICENCE 700 1- Tarkowski Andrej Department of Rheumatology, University of G¨oteborg, S-41346, G¨oteborg, Sweden aut NATIONALLICENCE 700 1- Porcelli Steven Lymphocyte Biology Section, Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, 02115, Boston, Massachusetts aut NATIONALLICENCE 700 1- Hanson Lars Department of Clinical Immunology, University of G¨oteborg, S-41346, G¨oteborg, Sweden aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/3(2000-05-01), 203-211 0271-9142 20:3<203 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer