caa a22 4500 475748344 CHVBK 20180406123519.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1023/A:1006637529995 doi (NATIONALLICENCE)springer-10.1023/A:1006637529995 Cytotoxic T-Lymphocyte Responses Elicited to Wilms' Tumor Gene WT1 Product by DNA Vaccination [Elektronische Daten] [Akihiro Tsuboi, Yoshihiro Oka, Hiroyasu Ogawa, Olga Elisseeva, Hanfen Li, Kotomi Kawasaki, Katsuyuki Aozasa, Tadamitsu Kishimoto, Keiko Udaka, Haruo Sugiyama] We recently have reported that Wilms' tumor gene WT1 is highly expressed not only in leukemias but also in various types of solid tumors and that WT1 protein is a novel tumor antigen against which cytotoxic T lymphocytes (CTLs) can be elicited by immunization with 9-mer WT1 peptides capable of binding to major histocompatibility complex (MHC) class I molecules. In the present study, plasmid DNA encoding murine full-length WT1 protein was injected intramuscularly into C57BL/6 mice. The mice vaccinated with the WT1 plasmid DNA elicited CTLs against the WT1 protein, and the CTLs specifically killed WT1-expressing tumor cells in a MHC class I-restricted manner. Furthermore, the vaccinated mice rejected the challenges of WT1-expressing tumor cells and survived with no signs of autoimmunity caused by the CTLs. These results demonstrated that vaccination with the WT1 plasmid DNA can elicit CTL responses specific for the WT1 protein, resulting in the acquisition of rejection activity against challenges of WT1-expressing tumor cells. This WT1 DNA vaccination may find clinical application for various types of solid tumors as well as leukemias. Plenum Publishing Corporation, 2000 WT1 nationallicence Wilms' nationallicence tumor gene nationallicence CTL nationallicence DNA vaccine nationallicence Tsuboi Akihiro Department of Molecular Medicine, Osaka University Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut Oka Yoshihiro Department of Molecular Medicine, Osaka University Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut Ogawa Hiroyasu Department of Molecular Medicine, Osaka University Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut Elisseeva Olga Department of Molecular Medicine, Osaka University Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut Li Hanfen Department of Clinical Laboratory Science, Osaka Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut Kawasaki Kotomi Department of Clinical Laboratory Science, Osaka Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut Aozasa Katsuyuki Department of Pathology, Osaka Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut Kishimoto Tadamitsu Osaka University, 1-1, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut Udaka Keiko PREST, JST, and Department of Biophysics, Kyoto University, Sakyo, Kyoto, Japan aut Sugiyama Haruo Department of Clinical Laboratory Science, Osaka Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/3(2000-05-01), 195-202 0271-9142 20:3<195 2000 20 10875 https://doi.org/10.1023/A:1006637529995 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1006637529995 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsuboi Akihiro Department of Molecular Medicine, Osaka University Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut NATIONALLICENCE 700 1- Oka Yoshihiro Department of Molecular Medicine, Osaka University Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut NATIONALLICENCE 700 1- Ogawa Hiroyasu Department of Molecular Medicine, Osaka University Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut NATIONALLICENCE 700 1- Elisseeva Olga Department of Molecular Medicine, Osaka University Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut NATIONALLICENCE 700 1- Li Hanfen Department of Clinical Laboratory Science, Osaka Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut NATIONALLICENCE 700 1- Kawasaki Kotomi Department of Clinical Laboratory Science, Osaka Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut NATIONALLICENCE 700 1- Aozasa Katsuyuki Department of Pathology, Osaka Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut NATIONALLICENCE 700 1- Kishimoto Tadamitsu Osaka University, 1-1, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut NATIONALLICENCE 700 1- Udaka Keiko PREST, JST, and Department of Biophysics, Kyoto University, Sakyo, Kyoto, Japan aut NATIONALLICENCE 700 1- Sugiyama Haruo Department of Clinical Laboratory Science, Osaka Medical School, 2-2, Yamada-Oka, 565-0871, Suita City, Osaka, Japan aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/3(2000-05-01), 195-202 0271-9142 20:3<195 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer