caa a22 4500 475748379 CHVBK 20180406123520.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1023/A:1006667703202 doi (NATIONALLICENCE)springer-10.1023/A:1006667703202 Immunoglobulin Isotypes of Anti-Myeloperoxidase and Anti-Lactoferrin Antibodies in Patients with Collagen Diseases [Elektronische Daten] [Hiroaki Chikazawa, Koji Nishiya, Akinori Matsumori, Kozo Hashimoto] To investigate the prevalence and clinical relevance of immunoglobulin (Ig) isotypes of antimyeloperoxidase (MPO) and antilactoferrin (LF) antibodies in collagen diseases, enzyme-linked immunosorbent assay was employed to detect the Ig isotypes of both antibodies. The purified proteins of MPO and LF were used as two major representative antigens for anti-neutrophil cytoplasmic antibodies (ANCA) with a perinuclear staining pattern by an indirect immunofluorescent technique. We examined 131 serum samples from 79 patients with rheumatoid arthritis (RA), 32 with systemic lupus erythematosus (SLE), 14 with progressive systemic sclerosis (PSS), 6 with polymyositis/dermatomyositis (PM/DM), and 5 with idiopathic crescentic glomerulonephritis who served as positive controls and 36 healthy subjects who served as controls. A limited number of patients with RA (4-10%), SLE (6-9%), and PSS (7-14%) but not PM/DM showed positive IgG or IgA anti-MPO antibody (MPO-ANCA) but not IgM MPO-ANCA. However, 10-20% of RA, 40-60% of SLE, 20-36% of PSS but none of the PM/DM patients showed positive IgG, IgA, or IgM anti-LF antibody (LF-ANCA). MPO- and LF-ANCA positivity in RA patients was correlated with markers of disease activity such as the erythrocyte sedimentation rate, C-reactive protein, and serum Ig levels. IgG LF-ANCA but not MPO-ANCA positivity in SLE patients also was correlated with the disease activity index but not with clinical features. Neither MPO- nor LF-ANCA positivity in PSS patients was correlated with any clinical features. Overall, both MPO- and LF-ANCA were found mainly in RA, SLE, and PSS patients but not in PM/DM patients. The Ig isotypes of MPO- and LF-ANCA frequently belonged to both IgG and IgA and rarely to the IgM class. Both MPO- and LF-ANCA positivity reflected disease activity in RA and SLE rather than specific organ involvement. Plenum Publishing Corporation, 2000 Immunoglobulin isotype nationallicence antimyeloperoxidase antibody nationallicence antilactoferrin antibody nationallicence collagen diseases nationallicence Chikazawa Hiroaki Second Department of Internal Medicine, Kochi Medical School, 783-8505, Nankoku City, Kochi, Japan aut Nishiya Koji Second Department of Internal Medicine, Kochi Medical School, 783-8505, Nankoku City, Kochi, Japan aut Matsumori Akinori Second Department of Internal Medicine, Kochi Medical School, 783-8505, Nankoku City, Kochi, Japan aut Hashimoto Kozo Second Department of Internal Medicine, Kochi Medical School, 783-8505, Nankoku City, Kochi, Japan aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/4(2000-07-01), 279-286 0271-9142 20:4<279 2000 20 10875 https://doi.org/10.1023/A:1006667703202 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1006667703202 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chikazawa Hiroaki Second Department of Internal Medicine, Kochi Medical School, 783-8505, Nankoku City, Kochi, Japan aut NATIONALLICENCE 700 1- Nishiya Koji Second Department of Internal Medicine, Kochi Medical School, 783-8505, Nankoku City, Kochi, Japan aut NATIONALLICENCE 700 1- Matsumori Akinori Second Department of Internal Medicine, Kochi Medical School, 783-8505, Nankoku City, Kochi, Japan aut NATIONALLICENCE 700 1- Hashimoto Kozo Second Department of Internal Medicine, Kochi Medical School, 783-8505, Nankoku City, Kochi, Japan aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/4(2000-07-01), 279-286 0271-9142 20:4<279 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer