caa a22 4500 475748441 CHVBK 20180406123520.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1023/A:1006671804110 doi (NATIONALLICENCE)springer-10.1023/A:1006671804110 The Mechanism of Mononuclear Cell Infiltration in Oral Lichen Planus: The Role of Cytokines Released from Keratinocytes [Elektronische Daten] [Tetsuya Yamamoto, Tomoko Nakane, Tokio Osaki] To clarify the pathogenesis of oral lichen planus (OLP), we investigated the roles of keratinocytes (KC) in mononuclear cell infiltration. When peripheral blood mononuclear cells (PBMC) obtained from healthy donors were cultured in the presence of culture supernatants of KC separated from the noninflamed gingivae (Nor-KC) and cheek mucosae of patients with OLP (OLP-KC), the number of migrated PBMC across monolayered human umbilical vein endothelial cells (HUVEC) were increased to about 1.3-fold and 1.5-fold of the control level, respectively, with increases of the expression of CD11a, CD11b, CD18, and CD49d on PBMC and intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial-leukocyte adhesion molecule-1 on HUVEC. The number of migrated PBMC was reduced to about 60% of the control level by pretreatment of PBMC with anti-CD11a or anti-CD18 MAb and reduced to about 70% by pretreatment of HUVEC with anti-CD54 MAb. The pretreatment of PBMC with genistein, H-7, wortmannin, or exoenzyme C3 decreased the migrated PBMC by about 70 to 90%. In agreement with these results, the culture supernatants of OLP-KC up-regulated tyrosine phosphorylation of 62-kDa, 70-kDa, and 102-kDa proteins, phosphatidylinositol-3 kinase, and protein kinase C activities and activated Rho protein level more so than did those of Nor-KC. Additionally, actin reorganization with the formation of membrane ruffles and lamellipodia was distinctly induced by the culture supernatants of OLP-KC. These results indicate that cytokines generated by KC transduce their signals in PBMC, up-regulating the expression of cell surface adhesion molecules and migration activity with reorganization of actin filaments. Plenum Publishing Corporation, 2000 Lichen planus nationallicence mononuclear cell infiltration nationallicence cytokines nationallicence adhesion molecules nationallicence signal transduction nationallicence Yamamoto Tetsuya Department of Oral Surgery, Kochi Medical School, Kohasu, Oko-cho, 783-8505, Nankoku-city, Kochi, Japan aut Nakane Tomoko Department of Oral Surgery, Kochi Medical School, Kohasu, Oko-cho, 783-8505, Nankoku-city, Kochi, Japan aut Osaki Tokio Department of Oral Surgery, Kochi Medical School, Kohasu, Oko-cho, 783-8505, Nankoku-city, Kochi, Japan aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/4(2000-07-01), 294-305 0271-9142 20:4<294 2000 20 10875 https://doi.org/10.1023/A:1006671804110 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1006671804110 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamamoto Tetsuya Department of Oral Surgery, Kochi Medical School, Kohasu, Oko-cho, 783-8505, Nankoku-city, Kochi, Japan aut NATIONALLICENCE 700 1- Nakane Tomoko Department of Oral Surgery, Kochi Medical School, Kohasu, Oko-cho, 783-8505, Nankoku-city, Kochi, Japan aut NATIONALLICENCE 700 1- Osaki Tokio Department of Oral Surgery, Kochi Medical School, Kohasu, Oko-cho, 783-8505, Nankoku-city, Kochi, Japan aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/4(2000-07-01), 294-305 0271-9142 20:4<294 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer