caa a22 4500 47574845X CHVBK 20180406123520.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1023/A:1017215720949 doi (NATIONALLICENCE)springer-10.1023/A:1017215720949 Mycobacterium tuberculosis-Secreted Protein Antigens: Immunogenicity in Baboons [Elektronische Daten] [Karen Pehler, Kathleen Brasky, Thomas Butler, Roberta Attanasio] The effective control of tuberculosis (TB) requires the development of improved vaccines. It is now well established that Mycobacterium tuberculosis-secreted antigens represent promising candidates to be included in subunit vaccine preparations. It also is accepted that studies in nonhuman primate models will be required to further develop these vaccine preparations. As a necessary step in this direction, we have assessed the immunogenicity of M. tuberculosis-secreted antigens in baboons. Animals received a total of three intramuscular injections consisting of M. tuberculosis culture filtrate proteins resuspended in an adjuvant formulation (MPL-SE) and were tested for development of specific antibody and cellular responses. All animals produced antibody and cellular proliferative responses in the absence of detectable delayed-type hypersensitivity reactions. Production of γ-interferon following stimulation of peripheral blood lymphocytes with culture filtrate proteins was either absent or present at low levels. Results from this study show that, although M. tuberculosis-secreted protein antigens are relatively safe and immunogenic in baboons, alternative immunization approaches must be identified for the induction of T-helper type 1 responses. Plenum Publishing Corporation, 2000 Tuberculosis nationallicence vaccines nationallicence secreted antigens nationallicence baboons nationallicence Pehler Karen aut Brasky Kathleen aut Butler Thomas aut Attanasio Roberta aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/4(2000-07-01), 306-316 0271-9142 20:4<306 2000 20 10875 https://doi.org/10.1023/A:1017215720949 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1017215720949 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pehler Karen aut NATIONALLICENCE 700 1- Brasky Kathleen aut NATIONALLICENCE 700 1- Butler Thomas aut NATIONALLICENCE 700 1- Attanasio Roberta aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/4(2000-07-01), 306-316 0271-9142 20:4<306 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer