caa a22 4500 475748514 CHVBK 20180406123520.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1023/A:1006620130114 doi (NATIONALLICENCE)springer-10.1023/A:1006620130114 Calorie Restriction Decreases Proinflammatory Cytokines and Polymeric Ig Receptor Expression in the Submandibular Glands of Autoimmune Prone (NZB × NZW)F1 Mice [Elektronische Daten] [Alagar Muthukumar, Christopher Jolly, Khaliquz Zaman, Gabriel Fernandes] Calorie restriction or fish oil (enriched in n-3 fatty acids) supplementation ameliorates glomerulonephritis and Sj¨ogren's syndrome lesions in (NZB × NZW)F1(B/W) mice. Enhanced proinflammatory cytokine expression and deposition of immune complexes are the important pathological events in the development of Sj¨ogren's syndrome. In the present study, we have examined the effect of calorie restriction and fish oil supplementation on the expression of key inflammatory cytokines [gamma interferon (INF-γ), interleukin-10 (IL-10), and IL-12] and polymeric immunoglobulin (Ig) receptor (pIgR) (receptor for IgA and IgM) and the secretion of Ig in the submandibular glands (SMG) of B/W mice. Weanling B/W mice were fed either ad libitum (AL) or calorie restricted (CR) (40% less calories than AL) diet supplemented with 5% corn oil (CO) or 5% fish oil (FO) until 4 or 9 months of age. The SMGs were removed and a portion of the tissue used for semiquantitive determinations of IFN-γ, IL-10, IL-12 (p40), and pIgR mRNA. The remaining SMG tissue was fragmented and cultured for 7 days and the culture supernatants assayed for IgA, IgM, and IgG2a levels by enzyme-linked immunosorbent assay. Results revealed a significant increase in the expression of IFN-γ, IL-10, and IL-12 mRNA with age in AL fed mice, whereas CR fed mice maintained their levels to near those seen in young animals regardless of the dietary fat. PIgR mRNA expression also remained unaltered in CR animals irrespective of age and dietary fat, while it was found significantly increased in AL fed mice. CR significantly inhibited the elevated levels of IgA and IgG2a seen in aged mice. Interestingly, CR also influenced the Ig level in young animals. In summary, these results indicate that amelioration of autoimmune disease by CR in B/W mice is possibly mediated by the lowered mRNA expression of IFN-γ, IL-10, IL-12, and pIgR and the reduced Ig secretion. Plenum Publishing Corporation, 2000 Calorie restriction nationallicence fish oil nationallicence cytokines nationallicence pIgR nationallicence lupus-prone mice nationallicence Muthukumar Alagar aut Jolly Christopher aut Zaman Khaliquz aut Fernandes Gabriel aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/5(2000-09-01), 354-361 0271-9142 20:5<354 2000 20 10875 https://doi.org/10.1023/A:1006620130114 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1006620130114 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Muthukumar Alagar aut NATIONALLICENCE 700 1- Jolly Christopher aut NATIONALLICENCE 700 1- Zaman Khaliquz aut NATIONALLICENCE 700 1- Fernandes Gabriel aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/5(2000-09-01), 354-361 0271-9142 20:5<354 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer