caa a22 4500 475748557 CHVBK 20180406123520.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1023/A:1006682413834 doi (NATIONALLICENCE)springer-10.1023/A:1006682413834 IL-4 and IFN-γ mRNA Induction in Human Peripheral Lymphocytes Specific for β-Lactam Antibiotics in Immediate or Delayed Hypersensitivity Reactions [Elektronische Daten] [Isabelle Gaspard, Marie-Th´er'sese Guinnepain, J´erome Laurent, Nicolas Bachot, Saadia Kerdine, Jacques Bertoglio, Marc Pallardy, Herv´e Lebrec] β-Lactam antibiotics elicit CD4+ and CD8+ T-cell-mediated immune responses that play a central role in allergic reactions. However, the involvement of a type 1- (Th1 or Tc1) or a type 2-like (Th2 or Tc2) differentiation in drug allergy remains unclear. We investigated the expression of interleukin 4 (IL-4) and interferon gamma (IFN-γ) mRNA by quantitative reverse transcription and polymerase chain reaction (RT-PCR) in patient-derived peripheral blood lymphocytes following specific in vitro stimulation. Samples were collected from a total of 19 patients who had developed immediate or delayed clinical manifestations of hypersensitivity to β-lactam and from 11 control subjects. Peripheral blood mononuclear cells (PBMCs) were stimulated with either free antibiotics or antibiotic-human serum albumin (HSA) conjugates. Specific induction of IFN-γ mRNA expression was observed in 11 of 11 allergic patients with immediate reactions, in 6 of 8 patients with delayed reactions, and in 4 of 11 control subjects. IL-4 mRNA expression was induced in 5 of 11 allergic individuals with immediate reactions but in none of the 8 patients with delayed responses and none of the 11 control subjects. IL-4 mRNA expression was only induced following activation with free drugs, while IFN-γ mRNA expression was predominantly induced in CD4+ T cells following stimulation with HSA-conjugated drugs. Immediate-type hypersensitivity to β-lactams was not associated with a pure type 2-like response when PBMCs were specifically stimulated in vitro: Some patients with well-documented history of β-lactam-induced immediate allergic reaction showed a high IFN-γ response. Contact dermatitis involved Tc1 and Th1 cells and other delayed hypersensitivity reactions to β-lactams were restricted to Th1 responses. Plenum Publishing Corporation, 2000 Hypersensitivity nationallicence allergy nationallicence cytokines nationallicence Th1/Th2 nationallicence Gaspard Isabelle Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut Guinnepain Marie-Th´er'sese Unit´e d'Allergologie de l'Institut Pasteur, 75015, Paris, France aut Laurent J´erome Unit´e d'Allergologie de l'Institut Pasteur, 75015, Paris, France aut Bachot Nicolas Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut Kerdine Saadia Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut Bertoglio Jacques Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut Pallardy Marc Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut Lebrec Herv´e Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/2(2000-03-01), 107-116 0271-9142 20:2<107 2000 20 10875 https://doi.org/10.1023/A:1006682413834 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1006682413834 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gaspard Isabelle Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut NATIONALLICENCE 700 1- Guinnepain Marie-Th´er'sese Unit´e d'Allergologie de l'Institut Pasteur, 75015, Paris, France aut NATIONALLICENCE 700 1- Laurent J´erome Unit´e d'Allergologie de l'Institut Pasteur, 75015, Paris, France aut NATIONALLICENCE 700 1- Bachot Nicolas Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut NATIONALLICENCE 700 1- Kerdine Saadia Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut NATIONALLICENCE 700 1- Bertoglio Jacques Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut NATIONALLICENCE 700 1- Pallardy Marc Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut NATIONALLICENCE 700 1- Lebrec Herv´e Facult´e de Pharmacie, INSERM U461, 5 rue JB Cl´ement, 92296, Ch^atenay-Malabry, France aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/2(2000-03-01), 107-116 0271-9142 20:2<107 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer