caa a22 4500 47574859X CHVBK 20180406123520.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1023/A:1006638631581 doi (NATIONALLICENCE)springer-10.1023/A:1006638631581 Vaccination Responses to Capsular Polysaccharides of Neisseria meningitidis and Haemophilus influenzae Type b in Two C2-Deficient Sisters: Alternative Pathway-Mediated Bacterial Killing and Evidence for a Novel Type of Blocking IgG [Elektronische Daten] [Barbro Selander, Helena K¨ayhty, Elisabeth Wedege, Eva Holmstr¨om, Lennart Truedsson, Claes S¨oderstr¨om, Anders Sj¨oholm] Meningitis caused by Neisseria meningitidis serogroup W-135 was diagnosed in a 14-year-old girl with a history of neonatal septicemia and meningitis caused by group B streptococci type III. C2 deficiency type I was found in the patient and her healthy sister. Both sisters were vaccinated with tetravalent meningococcal vaccine and a conjugate Haemophilus influenzae type b vaccine. Three main points emerged from the analysis. First, vaccination resulted in serum bactericidal responses demonstrating anticapsular antibody-mediated recruitment of the alternative pathway. Second, addition of C2 to prevaccination sera produced bactericidal activity in the absence of anticapsular antibodies, which suggested that the bactericidal action of antibodies to subcapsular antigens detected in the sera might strictly depend on the classical pathway. A third point concerned a previously unrecognized type of blocking activity. Thus, postvaccination sera of the healthy sister contained IgG that inhibited killing of serogroup W-135 in C2-deficient serum, and the deposition of C3 on enzyme-linked immunosorbent assay plates coated with purified W-135 polysaccharide. Our findings suggested blocking to be serogroup-specific and dependent on early classical pathway components. Retained opsonic activity probably supported postvaccination immunity despite blocking of the bactericidal activity. The demonstration of functional vaccination responses with recruitment of alternative pathway-mediated defense should encourage further trial of capsular vaccines in classical pathway deficiency states. Plenum Publishing Corporation, 2000 Complement nationallicence immunodeficiency diseases nationallicence infectious immunity nationallicence bacteria nationallicence vaccination nationallicence Selander Barbro Institute of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Lund, Sweden aut K¨ayhty Helena National Public Health Institute, Helsinki, Finland aut Wedege Elisabeth Department of Vaccinology, National Institute of Public Health, Oslo, Norway aut Holmstr¨om Eva Institute of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Lund, Sweden aut Truedsson Lennart Institute of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Lund, Sweden aut S¨oderstr¨om Claes Department of Infectious Diseases, University Hospital of Lund, Lund, Sweden aut Sj¨oholm Anders Institute of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Lund, Sweden aut Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/2(2000-03-01), 138-149 0271-9142 20:2<138 2000 20 10875 https://doi.org/10.1023/A:1006638631581 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1006638631581 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Selander Barbro Institute of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Lund, Sweden aut NATIONALLICENCE 700 1- K¨ayhty Helena National Public Health Institute, Helsinki, Finland aut NATIONALLICENCE 700 1- Wedege Elisabeth Department of Vaccinology, National Institute of Public Health, Oslo, Norway aut NATIONALLICENCE 700 1- Holmstr¨om Eva Institute of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Lund, Sweden aut NATIONALLICENCE 700 1- Truedsson Lennart Institute of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Lund, Sweden aut NATIONALLICENCE 700 1- S¨oderstr¨om Claes Department of Infectious Diseases, University Hospital of Lund, Lund, Sweden aut NATIONALLICENCE 700 1- Sj¨oholm Anders Institute of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Lund, Sweden aut NATIONALLICENCE 773 0- Journal of Clinical Immunology Kluwer Academic Publishers-Plenum Publishers 20/2(2000-03-01), 138-149 0271-9142 20:2<138 2000 20 10875 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer