caa a22 4500 475751914 CHVBK 20180406123526.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/PL00010046 doi (NATIONALLICENCE)springer-10.1007/PL00010046 The Role of Lipid Peroxidation on Gastric Mucosal Lesions Induced by Water-Immersion-Restraint Stress in Rats [Elektronische Daten] [Wei Ming Shian, Iwao Sasaki, Yasuhiko Kamiyama, Hiroo Naito, Seiki Matsuno, Teruo Miyazawa] This study was designed to determine the effect of water-immersion-restraint stress (WIRS) and pretreatment by reduced glutathione on both the production of gastric mucosal lesions and the content of gastric mucosal phosphatidylcholine hydroperoxide (PCOOH). Sprague-Dawley rats were divided into control and treatment groups (treated with reduced glutathione before stress). After graded durations of WIRS, the macroscopic ulcer index (UI) was measured and the PCOOH content was examined by a chemiluminescence-high performance liquid chromatography assay. The UI in the control group increased significantly in a time-dependent fashion. Elevation of the PCOOH level was observed in combination with the UI for up to 2 h of WIRS, but then showed a declining tendency. Pretreatment with reduced glutathione significantly lowered both the UI and the PCOOH level. Lipid peroxidation is probably involved in the pathogenesis of gastric injury induced by WIRS at least in the early phases. However, in the late phases, other mechanisms causing gastric mucosal lesions may also be involved. We therefore believe this study to be the first to accurately and quantitatively assess the degree of lipid peroxidation in the gastric mucosa as a result of stress.: Springer-Verlag Tokyo, 2000 Key Words: lipid peroxidation, gastric injury, water-immersion-restraint stress nationallicence Shian Wei Ming First Department of Surgery, School of Medicine, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut Sasaki Iwao First Department of Surgery, School of Medicine, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut Kamiyama Yasuhiko First Department of Surgery, School of Medicine, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut Naito Hiroo First Department of Surgery, School of Medicine, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut Matsuno Seiki First Department of Surgery, School of Medicine, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut Miyazawa Teruo Department of Applied Biology and Chemistry, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut https://doi.org/10.1007/PL00010046 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00010046 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shian Wei Ming First Department of Surgery, School of Medicine, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut NATIONALLICENCE 700 1- Sasaki Iwao First Department of Surgery, School of Medicine, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut NATIONALLICENCE 700 1- Kamiyama Yasuhiko First Department of Surgery, School of Medicine, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut NATIONALLICENCE 700 1- Naito Hiroo First Department of Surgery, School of Medicine, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut NATIONALLICENCE 700 1- Matsuno Seiki First Department of Surgery, School of Medicine, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut NATIONALLICENCE 700 1- Miyazawa Teruo Department of Applied Biology and Chemistry, Tohoku University, 1-1 Seiryocho, Aoba-ku, Sendai 980-8574, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer