caa a22 4500 475752236 CHVBK 20180406123527.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s005950070119 doi (NATIONALLICENCE)springer-10.1007/s005950070119 Heparin reduces serum levels of endothelin-1 and hepatic ischemia reperfusion injury in rabbits [Elektronische Daten] [Tadashi Matsumoto, Masahiko Yamaguchi, Hiroaki Kikuchi, Hiroshi Nakano, Takemasa Midorikawa, Kaoru Kumada, Minoru Takeda] Since the role of heparin in hepatic ischemia/ reperfusion (I/R) injury is still not fully understood, we investigated the effects of heparin on hepatic I/R injury in rabbits. Heparin was injected into rabbits after inducing partial hepatic ischemia for 1 h. Thereafter, the serum levels of endothelin-1 (ET-1) and liver transaminase, and tissue levels of oxidized and deoxidized hemoglobin (oxHb, deoxHb) in the reperfused liver were analyzed. Microscopic examinations were also performed. The increased serum levels of ET-1 and liver transaminase after reperfusion were significantly reduced by heparin (P>0.01). Hepatic ischemia reduced oxHb and increased deoxHb. Reperfusion with heparin immediately reduced deoxHb and increased oxHb, and thereafter the balance between the two kinds of Hb also recovered. However, reperfusion without heparin did not demonstrate any similar recovery, but instead gradually exacerbated the dissociation. Microscopically, heparin appeared to normalize I/R-induced activation of hepatic stellate cells which are the target cells for ET-1. These results suggest that heparin improves the hepatic I/R injury caused by sinusoidal microscirculatory disturbances partly via an inhibition of the ET-1 increase. Springer-Verlag, 2000 heparin nationallicence endothelin-1 nationallicence microcirculation nationallicence Matsumoto Tadashi Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut Yamaguchi Masahiko Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut Kikuchi Hiroaki Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut Nakano Hiroshi Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut Midorikawa Takemasa Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut Kumada Kaoru Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut Takeda Minoru Department of Biochemistry, Showa University Medical School, Tokyo, Japan aut Surgery Today Springer-Verlag 30/6(2000-06-01), 523-525 0941-1291 30:6<523 2000 30 595 https://doi.org/10.1007/s005950070119 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070119 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsumoto Tadashi Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Yamaguchi Masahiko Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Kikuchi Hiroaki Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Nakano Hiroshi Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Midorikawa Takemasa Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Kumada Kaoru Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Takeda Minoru Department of Biochemistry, Showa University Medical School, Tokyo, Japan aut NATIONALLICENCE 773 0- Surgery Today Springer-Verlag 30/6(2000-06-01), 523-525 0941-1291 30:6<523 2000 30 595 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer