caa a22 4500 47575235X CHVBK 20180406123527.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s005950070064 doi (NATIONALLICENCE)springer-10.1007/s005950070064 Human Skin Xenograft Rejection in CD45 Exon-6 Knockout Mice: The Implication of Involvement of a Direct Pathway [Elektronische Daten] [Yukihiro Tomita, Takayuki Uchida, Qi-Wei Zhang, Ichiro Shimizu, Toshiro Iwai, Masahiro Yoshikawa, Kenji Kishihara, Kikuo Nomoto, Hisataka Yasui] The results of previous studies indicate that only CD4+ T cells generated via the indirect pathway play an essential role in causing discordant skin xenograft rejection. The present study was conducted in an attempt to clarify further the roles of effector T cells generated via direct pathways on discordant xenograft rejection using CD45 exon-6 knockout (CD45−/−; C57BL/6 (B6): H-2b) mice. It has been strongly suggested that CD45 exon-6 knockout mice have profound impairment in T-cell functions via an indirect pathway. When human skin was grafted onto untreated normal C57BL/6 (B6; H-2b) mice, rejection occurred within 12 days; however, in the CD45 exon-6 knockout mice, the grafts lasted for slightly longer as in fully allogeneic C3H (H-2k) skin rejection, with a mean survival time ± SD of 19.4 ± 1.5 days and median survival times of 19 days. The difference in survival periods between the human and C3H skin grafts in the CD45 knockout mice was not statistically significant. Both CD4+ and CD8+ T cells seemed activated in the spleens of these CD45 exon-6 knockout mice 10 days after the human skin grafting. These results suggest that effector T cells generated via a direct pathway can cause discordant skin xenograft rejection, and that CD45 exon-6 knockout mice can generate effector T cells via a direct pathway to reject discordant skin xenografts, similarly to fully allogeneic skin allografts. Springer-Verlag, 2000 Key Words CD45 knockout mouse nationallicence Xenograft rejection nationallicence Indirect pathway nationallicence Tomita Yukihiro Department of Cardiovascular Surgery, Faculty of Medicine, and aut Uchida Takayuki Department of Cardiovascular Surgery, Faculty of Medicine, and aut Zhang Qi-Wei Department of Cardiovascular Surgery, Faculty of Medicine, and aut Shimizu Ichiro Department of Cardiovascular Surgery, Faculty of Medicine, and aut Iwai Toshiro Department of Cardiovascular Surgery, Faculty of Medicine, and aut Yoshikawa Masahiro Department of Immunology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut Kishihara Kenji Department of Immunology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut Nomoto Kikuo Department of Immunology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut Yasui Hisataka Department of Cardiovascular Surgery, Faculty of Medicine, and aut https://doi.org/10.1007/s005950070064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070064 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tomita Yukihiro Department of Cardiovascular Surgery, Faculty of Medicine, and aut NATIONALLICENCE 700 1- Uchida Takayuki Department of Cardiovascular Surgery, Faculty of Medicine, and aut NATIONALLICENCE 700 1- Zhang Qi-Wei Department of Cardiovascular Surgery, Faculty of Medicine, and aut NATIONALLICENCE 700 1- Shimizu Ichiro Department of Cardiovascular Surgery, Faculty of Medicine, and aut NATIONALLICENCE 700 1- Iwai Toshiro Department of Cardiovascular Surgery, Faculty of Medicine, and aut NATIONALLICENCE 700 1- Yoshikawa Masahiro Department of Immunology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut NATIONALLICENCE 700 1- Kishihara Kenji Department of Immunology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut NATIONALLICENCE 700 1- Nomoto Kikuo Department of Immunology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut NATIONALLICENCE 700 1- Yasui Hisataka Department of Cardiovascular Surgery, Faculty of Medicine, and aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer