caa a22 4500 475752473 CHVBK 20180406123528.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s005950070062 doi (NATIONALLICENCE)springer-10.1007/s005950070062 The Differential Cardioprotective Effects of Nucleoside Transport Inhibitor on Moderate and Deep Hypothermic Ischemia with Cold Cardioplegia [Elektronische Daten] [Takahiro Nishida, Munetaka Masuda, Yasuo Kanegae, Kazuyuki Miyamoto, Ryuji Tominaga, Yoshito Kawachi, Hisataka Yasui] The differences in the cardioprotective effects of nucleoside transport inhibitor (NTI) which is known to accumulate endogenous adenosine, on moderate and deep hypothermic ischemia, were examined. Using the Langendorff model, isolated, perfused rat hearts were arrested with cold cardioplegia and subjected to 90 min of global ischemia followed by 40 min of reperfusion. The temperature during ischemia was maintained at either 10°C (groups 1 and 2) or 25°C (groups 3 and 4). In groups 2 and 4, NTI in the form of R75231, 1 mg/l, was added to the cardioplegic solution. The intramyocardial adenosine triphosphate content at the end of ischemia was significantly lower in the moderate hypothermia groups than in the deep hypothermia groups. In the moderate hypothermia groups, NTI significantly enhanced the adenosine accumulation at the end of ischemia. Moreover, the recovery of both the contractile function and coronary flow rate in group 4 was superior to that in group 3, and was similar to those in groups 1 and 2. The addition of NTI to the cardioplegic solution generated a sufficient cardioprotective effect in moderate hypothermic ischemia, but not in deep hypothermic ischemia. The mechanism of this discrepancy is attributed to the differences in the levels of endogenous adenosine accumulated during ischemia. Springer-Verlag, 2000 Key Words Heart nationallicence Adenosine nationallicence Ischemia nationallicence Reperfusion nationallicence Cardioprotection nationallicence Adenosine triphosphate nationallicence Nishida Takahiro Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut Masuda Munetaka Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut Kanegae Yasuo Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut Miyamoto Kazuyuki Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut Tominaga Ryuji Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut Kawachi Yoshito Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut Yasui Hisataka Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut https://doi.org/10.1007/s005950070062 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070062 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishida Takahiro Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut NATIONALLICENCE 700 1- Masuda Munetaka Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut NATIONALLICENCE 700 1- Kanegae Yasuo Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut NATIONALLICENCE 700 1- Miyamoto Kazuyuki Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut NATIONALLICENCE 700 1- Tominaga Ryuji Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut NATIONALLICENCE 700 1- Kawachi Yoshito Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut NATIONALLICENCE 700 1- Yasui Hisataka Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer