caa a22 4500 475752643 CHVBK 20180406123528.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s005950070102 doi (NATIONALLICENCE)springer-10.1007/s005950070102 Changes in the expression of lipid-mediated signal-transducing enzymes in the rat Liver after partial hepatectomy [Elektronische Daten] [Atsushi Watanabe, Shigeru Nakashima, Takahito Adachi, Shigetoyo Saji, Yoshinori Nozawa] Prostaglandins (PGs), metabolites of arachidonic acid, and other lipid mediators produced by phospholipases C (PLC) and D (PLD) are thought to play important roles in hepatocyte proliferation. The present study examined lipid-mediated signaling in the rat liver after partial hepatectomy (PH). Rats were killed 1-48 h after 70% PH and the remaining liver tissue was removed. The mRNA and protein levels of some signaling molecules were measured by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting, respectively. The levels of hepatocyte growth factor (HGF) mRNA showed a biphasic change, peaking 3 h and 9 h after PH. The expression of PLCδ4 peaked at 12 h, but no significant changes in the expression of PLCβ1 and PLCγ1 were seen after PH. T he enzymes involved in PG production, namely, the expression of cytosolic PLA2 and cyclooxygenase 1 (COX1), remained constant after PH. However, the mRNA of COX2 increased transiently at 3 h, and Western blot analysis showed an increase in COX2 protein at 12 h. The expression of PLD1b peaked at 9 h and PLD1a at 12 h, whereas the expression of PLD2 remained consistent for 24 h. These results suggest that transcriptional controls may act for PLCδ4, PLD1a/b, and COX2 during hepatocyte regeneration after PH. Springer-Verlag, 2000 Partial hepatectomy nationallicence Signal transduction nationallicence Phospholipase C nationallicence Phospholipase A2 nationallicence Cyclooxygenase nationallicence Phospholipase D nationallicence Hepatocyte growth factor nationallicence Watanabe Atsushi Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasamachi, 500-8705, Gifu, Japan aut Nakashima Shigeru Department of Biochemistry, Gifu University School of Medicine, 40 Tsukasamachi, 500-8705, Gifu, Japan aut Adachi Takahito Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasamachi, 500-8705, Gifu, Japan aut Saji Shigetoyo Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasamachi, 500-8705, Gifu, Japan aut Nozawa Yoshinori Department of Biochemistry, Gifu University School of Medicine, 40 Tsukasamachi, 500-8705, Gifu, Japan aut Surgery Today Springer-Verlag 30/7(2000-07-01), 622-630 0941-1291 30:7<622 2000 30 595 https://doi.org/10.1007/s005950070102 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070102 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Watanabe Atsushi Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasamachi, 500-8705, Gifu, Japan aut NATIONALLICENCE 700 1- Nakashima Shigeru Department of Biochemistry, Gifu University School of Medicine, 40 Tsukasamachi, 500-8705, Gifu, Japan aut NATIONALLICENCE 700 1- Adachi Takahito Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasamachi, 500-8705, Gifu, Japan aut NATIONALLICENCE 700 1- Saji Shigetoyo Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasamachi, 500-8705, Gifu, Japan aut NATIONALLICENCE 700 1- Nozawa Yoshinori Department of Biochemistry, Gifu University School of Medicine, 40 Tsukasamachi, 500-8705, Gifu, Japan aut NATIONALLICENCE 773 0- Surgery Today Springer-Verlag 30/7(2000-07-01), 622-630 0941-1291 30:7<622 2000 30 595 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer