caa a22 4500 475752678 CHVBK 20180406123528.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s005950070101 doi (NATIONALLICENCE)springer-10.1007/s005950070101 The significance of MMP-1 and MMP-2 in peritoneal disseminated metastasis of gastric cancer [Elektronische Daten] [Kazuki Mizutani, Kikuo Kofuji, Kazuo Shirouzu] The purpose of this study was to clarify the role of the matrix metalloproteinases (MMPs), collagenase (MMP-1), and gelatinase A (MMP-2), both of which are known to be involved in the development of gastric cancer, in peritoneal dissemination. The concentrations of MMP-1 and MMP-2 in the supernatant of mixed culture simulated peritoneal dissemination were measured in vitro with mesothelial cells and cancer cells using an enzyme-linked immunosorbent assay. The concentration of MMP-1 increased significantly after the contact culture was mixed with these two cells, in comparison with the non-contact mixed culture or the mesothelial cell culture alone. These results demonstrate that the production of MMP-1 derived from mesothelial cells was increased by contact with cancer cells. To clarify the effect of MMP-1 and MMP-2 on gastric cancer invasion, an invasion assay using matrigel was performed. After treatment with anti-MMP-1 monoclonal antibody (mAb) or anti-MMP-2 mAb, the number of matrigel-penetrating cancer cells was significantly reduced, indicating that MMP-1 and MMP-2 derived from mesothelial cells had a strong reaction to gastric cancer invasion. In conclusion, as MMP-1 showed a paracrine-like action responding to stimulus from cancer cells, it seemed to play an important role in the progression of peritoneal dissemination. Springer-Verlag, 2000 MMP-1 nationallicence MMP-2 nationallicence Peritoneal dissemination nationallicence Gastric cancer nationallicence Mizutani Kazuki Department of Surgery, Kurume University, School of Medicine, 67 Asahi-machi, Kurume, 830-0011, Fukuoka, Japan aut Kofuji Kikuo aut Shirouzu Kazuo aut Surgery Today Springer-Verlag 30/7(2000-07-01), 614-621 0941-1291 30:7<614 2000 30 595 https://doi.org/10.1007/s005950070101 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070101 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mizutani Kazuki Department of Surgery, Kurume University, School of Medicine, 67 Asahi-machi, Kurume, 830-0011, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kofuji Kikuo aut NATIONALLICENCE 700 1- Shirouzu Kazuo aut NATIONALLICENCE 773 0- Surgery Today Springer-Verlag 30/7(2000-07-01), 614-621 0941-1291 30:7<614 2000 30 595 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer