caa a22 4500 475752759 CHVBK 20180406123529.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s005950070005 doi (NATIONALLICENCE)springer-10.1007/s005950070005 Immunocytochemical Analysis of Cellular Infiltrates in Human Appendicitis [Elektronische Daten] [Takayuki Kuga, Satoshi Taniguchi, Takashi Inoue, Nobuya Zempo, Kensuke Esato] This study was conducted to determine the immunologic cellular composition in human ap-pendicitis and its association with the development of perforated appendicitis. Appendiceal specimens from 27 patients with acute appendicitis were immunostained to detect lymphocyte surface markers. Moreover, the lymphocyte surface markers of peripheral blood were analyzed by laser flow cytometry in 12 patients. Helper T lymphocytes (CD4) were present in all the patients, while B lymphocytes (CD19), natural killer (NK) cells (CD56), and cytotoxic T lymphocytes (CD8) were present in 7 (70%), 10 (100%), and 9 patients (90%) with perforated appendicitis, and in 12 (63.2%), 10 (58.8%), and 6 (54.5%) patients without perforation, respectively. There were significant differences between the patients with a perforated appendix and those without perforation, in the positivity rate for CD8 and CD56 cells (P < 0.05). The number of cells positive for CD56, being NK cells, in the blood from the patients with perforation was significantly lower than that in the blood from those without perforation (P < 0.05). The infiltration of a greater number of cytotoxic T lymphocytes and NK cells was observed in the appendices from patients with perforated appendicitis than in those from patients with nonperforated appendicitis. Springer-Verlag, 2000 Key words Acute appendicitis nationallicence Perforated appendicitis nationallicence Lymphocyte homing nationallicence Cytotoxic T lymphocyte nationallicence Natural killer cell nationallicence Kuga Takayuki First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, JP aut Taniguchi Satoshi First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, JP aut Inoue Takashi First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, JP aut Zempo Nobuya First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, JP aut Esato Kensuke First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, JP aut https://doi.org/10.1007/s005950070005 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070005 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuga Takayuki First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, JP aut NATIONALLICENCE 700 1- Taniguchi Satoshi First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, JP aut NATIONALLICENCE 700 1- Inoue Takashi First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, JP aut NATIONALLICENCE 700 1- Zempo Nobuya First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, JP aut NATIONALLICENCE 700 1- Esato Kensuke First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer