caa a22 4500 475752775 CHVBK 20180406123529.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s005950070004 doi (NATIONALLICENCE)springer-10.1007/s005950070004 Lymph Node Metastasis-Related Carbohydrate Epitopes of Gastric Cancer with Submucosal Invasion [Elektronische Daten] [Yoshinao Takano, Yasushi Teranishi, Shinya Terashima, Ryoichi Motoki, Takanori Kawaguchi] This study was conducted to examine the lymph node metastasis-related carbohydrate epitopes of cancer cells in primary lesions of gastric cancer with submucosal invasion (sm gastric cancer). A total of 118 formalin-fixed and paraffin-embedded surgical specimens were studied. Carbohydrate epitopes were de-tected histochemically using 17 kinds of biotin-labeled lectins and three kinds of monoclonal antibodies (mAbs) against Thomsen-Friedenreich antigen (HB-T1), Tn (HB-Tn1), and sialyl Tn antigens (HB-STn1). The node-positive group showed significantly lower reactivities with Ricinus communis I (RCA-I), Lotus tetragonolobus (Lotus), Triticum vulgaris (WGA), and Concanavalia ensiformis (ConA), and higher reac-tivities with HB-STn1. When sm gastric cancer was classified into differentiated and undifferentiated types, lower reactivities with RCA-I and ConA were observed in the differentiated type cancers, whereas lower re-activities with Lotus, WGA, and higher reactivity with HB-STn1 were observed in the undifferentiated type cancers. Nodal status was able to be predicted with high precision by a combination of these carbohydrates and histological subtypes. Since neuraminidase treatment rarely changed the reactivity of cancer cells with these lectins, we conclude that the loss of certain kinds of carbohydrates that are restricted by histological subtypes is related to lymph node metastasis in sm gastric cancer. Springer-Verlag, 2000 Key words Early gastric cancer nationallicence Lymph node metastasis nationallicence Carbohydrate nationallicence Lectin nationallicence Sialyl Tn antigen nationallicence Takano Yoshinao Department of Surgery, Southern Tohoku Research Institute of Neuroscience, 7-115 Yatsuyamada, Koriyama-shi, Fukushima 963-8563, Japan, JP aut Teranishi Yasushi Department of Surgery, Southern Tohoku Research Institute of Neuroscience, 7-115 Yatsuyamada, Koriyama-shi, Fukushima 963-8563, Japan, JP aut Terashima Shinya First Department of Surgery and aut Motoki Ryoichi First Department of Surgery and aut Kawaguchi Takanori Second Department of Pathology, Fukushima Medical University, School of Medicine, Hikarigaoka, Fukushima-shi, Fukushima 960-1247, Japan, JP aut https://doi.org/10.1007/s005950070004 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070004 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takano Yoshinao Department of Surgery, Southern Tohoku Research Institute of Neuroscience, 7-115 Yatsuyamada, Koriyama-shi, Fukushima 963-8563, Japan, JP aut NATIONALLICENCE 700 1- Teranishi Yasushi Department of Surgery, Southern Tohoku Research Institute of Neuroscience, 7-115 Yatsuyamada, Koriyama-shi, Fukushima 963-8563, Japan, JP aut NATIONALLICENCE 700 1- Terashima Shinya First Department of Surgery and aut NATIONALLICENCE 700 1- Motoki Ryoichi First Department of Surgery and aut NATIONALLICENCE 700 1- Kawaguchi Takanori Second Department of Pathology, Fukushima Medical University, School of Medicine, Hikarigaoka, Fukushima-shi, Fukushima 960-1247, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer