caa a22 4500 475752813 CHVBK 20180406123529.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s005950070008 doi (NATIONALLICENCE)springer-10.1007/s005950070008 Therapeutic Effect of TNP-470 on Spontaneous Liver Metastasis of Colon Tumors in the Rabbit [Elektronische Daten] [Jun Sano, Yasuyuki Sugiyama, Katsuyuki Kunieda, Bun Sano, Shigetoyo Saji] Even though angiogenesis inhibitor is thought to be one of the promising agents in tumor dormancy therapy, its optimal administration is still unknown. Therefore, the efficient protocol using TNP-470 was examined regarding its treatment affect against spontaneous liver metastases of colon tumors in the rabbit. A spontaneous liver metastases model was established in the rabbit by the inoculation of VX-2 tumors into the subserosal space of the colon. The therapeutic effect of TNP-470 was then investigated by monitoring both the number of metastatic nodules in the liver and the microvessel density (MVD) in the tumor by immuno-histochemical staining using anti-CD31 monoclonal antibody. TNP-470 did not show any effect on the primary tumor. It was able to reduce the metastatic spread to liver when it was administered at the microscopic metastatic stage. Treatment at this stage, however, was not able to sufficiently control the disease. These results indicated that TNP-470 could efficiently cause the tumor to enter into a dormant state by inhibiting angiogenesis when it was used at the initial stage of the metastatic process in the liver. Regarding its clinical application, TNP-470 might be useful for preventing the metachronous liver metastases of colorectal cancer when it is administered as adjuvant therapy after curative surgery. Springer-Verlag, 2000 Key words TNP-470 nationallicence Angiogenesis inhibitor nationallicence Tumor dormancy therapy nationallicence Spontaneous liver metastasis nationallicence VX-2 tumor nationallicence Sano Jun Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan, JP aut Sugiyama Yasuyuki Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan, JP aut Kunieda Katsuyuki Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan, JP aut Sano Bun Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan, JP aut Saji Shigetoyo Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan, JP aut https://doi.org/10.1007/s005950070008 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sano Jun Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan, JP aut NATIONALLICENCE 700 1- Sugiyama Yasuyuki Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan, JP aut NATIONALLICENCE 700 1- Kunieda Katsuyuki Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan, JP aut NATIONALLICENCE 700 1- Sano Bun Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan, JP aut NATIONALLICENCE 700 1- Saji Shigetoyo Second Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer