caa a22 4500 475753232 CHVBK 20180406123530.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s005950070042 doi (NATIONALLICENCE)springer-10.1007/s005950070042 The cytotoxic effects of bile acids in crude bile on human pancreatic cancer cell lines [Elektronische Daten] [Yi Lu, Masahiko Onda, Eiji Uchida, Susumu Yamamura, Ken Yanagi, Akira Matsushita, Tadashi Kobayashi, Munehisa Fukuhara, Kuniharu Aida, Takashi Tajiri] Pancreatic cancer frequently causes extrahepatic cholestasis. To identify the direct effects of bile acids in jaundiced serum on pancreatic cancer, the proliferation of PANC-1 and MIA PaCa-2 cells as well as the ultrastructural alteration of PANC-1 cells cultured in crude bile modified media were studied. The growth of these cells in the RPMI-1640 media with or without 1%, 2%, and 4% of the refined crude bile was assessed after 48 and 96 h of incubation. The ultrastructure of PANC-1 cells was investigated by scanning and transmission electron microscopy after 24 and 48 h of incubation. The proliferation of both cell lines in the bile-treated media was greatly inhibited. The inhibitory rates of bile on PANC-1 ranged from 24.1%±3.3% to 66.9%±6.6% (P < 0.01) and those on MIA PaCa-2 ranged from 16.7%±3.8% to 50.7%±5.5%. (P < 0.01). When the bile-added media were replaced, the cells were able to restore their proliferating ability. The PANC-1 cells incubated in the bile-supplied media indicated that the mirovilli, mitochondria, and other organelles had thus been injured. These results suggest that bile acids appear to inhibit the proliferation of PANC-1 and MIA PaCa-2 cells, and the probable inhibitory mechanism is mainly considered to be due to the cytotoxicity of such bile acids. Springer-Verlag, 2000 Bile acid nationallicence Extrahepatic cholestasis nationallicence Cytotoxicity nationallicence Pancreatic cancer cell line nationallicence Lu Yi First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut Onda Masahiko First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut Uchida Eiji First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut Yamamura Susumu First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut Yanagi Ken First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut Matsushita Akira First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut Kobayashi Tadashi First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut Fukuhara Munehisa First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut Aida Kuniharu First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut Tajiri Takashi First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut Surgery Today Springer-Verlag 30/10(2000-10-01), 903-909 0941-1291 30:10<903 2000 30 595 https://doi.org/10.1007/s005950070042 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070042 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Yi First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut NATIONALLICENCE 700 1- Onda Masahiko First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut NATIONALLICENCE 700 1- Uchida Eiji First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamamura Susumu First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut NATIONALLICENCE 700 1- Yanagi Ken First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsushita Akira First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut NATIONALLICENCE 700 1- Kobayashi Tadashi First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut NATIONALLICENCE 700 1- Fukuhara Munehisa First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut NATIONALLICENCE 700 1- Aida Kuniharu First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut NATIONALLICENCE 700 1- Tajiri Takashi First Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113-0022, Tokyo, Japan aut NATIONALLICENCE 773 0- Surgery Today Springer-Verlag 30/10(2000-10-01), 903-909 0941-1291 30:10<903 2000 30 595 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer