caa a22 4500 475753275 CHVBK 20180406123530.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s005950050599 doi (NATIONALLICENCE)springer-10.1007/s005950050599 Limitations of Exogenous L -Arginine in Exerting a Cytoprotective Effect on Hepatic Ischemia/Reperfusion Injury [Elektronische Daten] [Takao Higa, Masayuki Shiraishi, Toure Mamadi, Kaoru Taira, Takashi Oshiro, Eiji Nozato, Masayoshi Nagahama, Yoshihiro Muto] To test whether or not the L-arginine/nitric oxide (NO) pathway induces a protective effect, we investigated the effect of exogenous L-arginine on hepatic ischemia/reperfusion (I/R) injury, using ex vivo perfusion of the isolated rat liver. The rat liver was removed and preserved in cold saline for 60 min, followed by 120 min of reperfusion with oxygenated perfusate at 37°C. Either 600 mg/kg of L-arginine (groups 1 and 4), D-arginine (group 2), N G-nitro-L-arginine methyl ester (L-NAME) (group 3), or saline (group 5) were administered through the portal vein starting from 5 min before reperfusion to 5 min after reperfusion. In group 4, 600 mg/kg of L-NAME was preadministered at 10 min prior to the administration of L-arginine. The intrahepatic nitric oxide (NO) levels showed only a temporal elevation (227% ± 70% of the pre-reperfusion levels at 5 min) after reperfusion in group 1. Pretreatment with L-NAME suppressed the elevation of the NO levels immediately after reperfusion in group 4. The lactate dehydrogenase release to the effluent perfusate significantly decreased and the histological findings showed that the sinusoidal damage observed after reperfusion was mitigated in group 1 more than in the other groups. These results thus suggest that exogenous L-arginine produced a relatively small amount of NO and therefore resulted in a slight decrease of hepatic I/R injury.: Springer-Verlag Tokyo, 2000 Key Words: exogenous L-arginine, ischemia/reperfusion injury, ex vivo, liver nationallicence Higa Takao First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut Shiraishi Masayuki First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut Mamadi Toure First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut Taira Kaoru First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut Oshiro Takashi First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut Nozato Eiji First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut Nagahama Masayoshi First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut Muto Yoshihiro First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut https://doi.org/10.1007/s005950050599 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950050599 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Higa Takao First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut NATIONALLICENCE 700 1- Shiraishi Masayuki First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut NATIONALLICENCE 700 1- Mamadi Toure First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut NATIONALLICENCE 700 1- Taira Kaoru First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut NATIONALLICENCE 700 1- Oshiro Takashi First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut NATIONALLICENCE 700 1- Nozato Eiji First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut NATIONALLICENCE 700 1- Nagahama Masayoshi First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut NATIONALLICENCE 700 1- Muto Yoshihiro First Department of Surgery, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer