caa a22 4500 475753461 CHVBK 20180406123531.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s005950070081 doi (NATIONALLICENCE)springer-10.1007/s005950070081 Correlation between the expression of methionine adenosyltransferase and the stages of human colorectal carcinoma [Elektronische Daten] [Koji Ito, Satoru Ikeda, Naosuke Kojima, Mitsutaka Miura, Keiko Shimizu-Saito, Iku Yamaguchi, Iwao Katsuyama, Katsuhiro Sanada, Takehisa Iwai, Haruki Senoo, Saburo Horikawa] Methionine adenosyltransferase (MAT) catalyzes the synthesis of S-adenosylmethionine (AdoMet) from ATP and l-methionine. AdoMet is the major methyl donor in most transmethylation reactions in vivo, and it is also the propylamino donor in the biosynthesis of polyamines. In the present study, we assessed MAT activity in human colons with colorectal carcinoma and the values were compared with those of morphologically normal adjacent mucosa. Higher levels of MAT activity were observed in the colorectal carcinoma than in the normal colon. The ratio of MAT activity in tumor tissue versus normal tissue seemed to be correlated well will the stage of the colorectal tumor. Furthermore, immunoblot analysis showed that the high levels of MAT activity observed in colorectal carcinoma were due to the increased amounts of MAT protein. Immunohistochemical analysis revealed that MAT was most abundant in goblet cells, particularly in granules in the supranuclear area of these cells. In the colorectal carcinoma tissues, MAT was strongly stained in the cancerous cells and localized in granules in the supranuclear region. The results of this preliminary study suggest that determination of the relative ratio of MAT activity in both normal and tumor regions in human colorectal carcinoma could be a clinically useful tool for determining the stage of malignancy of colorectal carcinomas. Springer-Verlag, 2000 Methionine adenosyltransferase nationallicence Colorectal nationallicence adenocarcinoma nationallicence Colon nationallicence Immunohistochemistry nationallicence Ito Koji Department of Surgery, Tsuchiura Kyodo Hospital, 300-0053, Tsuchiura, Japan aut Ikeda Satoru Department of Pathology, Tsuchiura Kyodo Hospital, 300-0053, Tsuchiura, Japan aut Kojima Naosuke Department of Anatomy, Akita University School of Medicine, 010-0000, Akita, Japan aut Miura Mitsutaka Department of Anatomy, Akita University School of Medicine, 010-0000, Akita, Japan aut Shimizu-Saito Keiko Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda-ku, 101-0062, Tokyo, Japan aut Yamaguchi Iku Biochemical and Pharmaceutical Laboratories Inc., Tondabayashi, 584-0023, Osaka, Japan aut Katsuyama Iwao Biochemical and Pharmaceutical Laboratories Inc., Tondabayashi, 584-0023, Osaka, Japan aut Sanada Katsuhiro Department of Surgery, Tsuchiura Kyodo Hospital, 300-0053, Tsuchiura, Japan aut Iwai Takehisa First Department of Surgery, Faculty of Medicine, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, 113-8519, Tokyo, Japan aut Senoo Haruki Department of Anatomy, Akita University School of Medicine, 010-0000, Akita, Japan aut Horikawa Saburo Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda-ku, 101-0062, Tokyo, Japan aut Surgery Today Springer-Verlag 30/8(2000-08-01), 706-710 0941-1291 30:8<706 2000 30 595 https://doi.org/10.1007/s005950070081 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070081 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ito Koji Department of Surgery, Tsuchiura Kyodo Hospital, 300-0053, Tsuchiura, Japan aut NATIONALLICENCE 700 1- Ikeda Satoru Department of Pathology, Tsuchiura Kyodo Hospital, 300-0053, Tsuchiura, Japan aut NATIONALLICENCE 700 1- Kojima Naosuke Department of Anatomy, Akita University School of Medicine, 010-0000, Akita, Japan aut NATIONALLICENCE 700 1- Miura Mitsutaka Department of Anatomy, Akita University School of Medicine, 010-0000, Akita, Japan aut NATIONALLICENCE 700 1- Shimizu-Saito Keiko Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda-ku, 101-0062, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamaguchi Iku Biochemical and Pharmaceutical Laboratories Inc., Tondabayashi, 584-0023, Osaka, Japan aut NATIONALLICENCE 700 1- Katsuyama Iwao Biochemical and Pharmaceutical Laboratories Inc., Tondabayashi, 584-0023, Osaka, Japan aut NATIONALLICENCE 700 1- Sanada Katsuhiro Department of Surgery, Tsuchiura Kyodo Hospital, 300-0053, Tsuchiura, Japan aut NATIONALLICENCE 700 1- Iwai Takehisa First Department of Surgery, Faculty of Medicine, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, 113-8519, Tokyo, Japan aut NATIONALLICENCE 700 1- Senoo Haruki Department of Anatomy, Akita University School of Medicine, 010-0000, Akita, Japan aut NATIONALLICENCE 700 1- Horikawa Saburo Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda-ku, 101-0062, Tokyo, Japan aut NATIONALLICENCE 773 0- Surgery Today Springer-Verlag 30/8(2000-08-01), 706-710 0941-1291 30:8<706 2000 30 595 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer