caa a22 4500 475753682 CHVBK 20180406123532.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s005950050615 doi (NATIONALLICENCE)springer-10.1007/s005950050615 The effect of intraoperative colonic lavage with N G -nitro- l -arginine methyl ester ( l -NAME) on anastomotic healing in the presence of left-sided colonic obstruction in the rat [Elektronische Daten] [Yesim Erbil, Alper Çalis, Eren Berber, Selçuk Mercan] The effect of intraoperative colonic lavage withN G -nitro-l-arginine methyl ester (l-NAME) on the healing of colonic anastomosis in the presence of a left-sided obstruction in the rat was investigated. Left-sided colonic obstruction was created in 144 Wistar rats. The obstruction site was excised 24 h later and anastomosis was performed after either no irrigation or colonic lavage with either saline, povidone iodine (PI), short-chain fatty acids (SCFA),l-NAME, or glutamine, in 24 animals each. Animals were killed on days 3 and 6, and a 4-cm colonic segment with the anastomosis at the center was excised. Bursting pressure (BP) and hydroxyproline (HP) content were measured. In the saline, PI, and SCFA groups, BP was higher (P<0.05,P<0.05, andP<0.001, respectively) and HP concentration was similar compared with controls. Both the BP and HP concentrations were higher in the glutamine group compared with controls (P<0.001). BP was lower (P<0.05) and HP concentration was similar in thel-NAME group compared with the control group. Colonocyte nutrition and tissue perfusion are the mainstays of anastomotic healing. Intraoperative colonic lavage withl-NAME suppresses colonic anastomotic healing in the presence of a left-sided obstruction. Springer-Verlag, 2000 intestinal obstruction nationallicence irrigation nationallicence N G -nitro-larginine methyl ester nationallicence anastomosis nationallicence wound healing nationallicence pressure nationallicence rat nationallicence Erbil Yesim Department of General Surgery, Istanbul University, Istanbul Faculty of Medicine, Çapa, 34390, Istanbul, Turkey aut Çalis Alper Department of General Surgery, Istanbul University, Istanbul Faculty of Medicine, Çapa, 34390, Istanbul, Turkey aut Berber Eren Department of General Surgery, Istanbul University, Istanbul Faculty of Medicine, Çapa, 34390, Istanbul, Turkey aut Mercan Selçuk Department of General Surgery, Istanbul University, Istanbul Faculty of Medicine, Çapa, 34390, Istanbul, Turkey aut Surgery Today Springer-Verlag 30/5(2000-05-01), 421-425 0941-1291 30:5<421 2000 30 595 https://doi.org/10.1007/s005950050615 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950050615 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Erbil Yesim Department of General Surgery, Istanbul University, Istanbul Faculty of Medicine, Çapa, 34390, Istanbul, Turkey aut NATIONALLICENCE 700 1- Çalis Alper Department of General Surgery, Istanbul University, Istanbul Faculty of Medicine, Çapa, 34390, Istanbul, Turkey aut NATIONALLICENCE 700 1- Berber Eren Department of General Surgery, Istanbul University, Istanbul Faculty of Medicine, Çapa, 34390, Istanbul, Turkey aut NATIONALLICENCE 700 1- Mercan Selçuk Department of General Surgery, Istanbul University, Istanbul Faculty of Medicine, Çapa, 34390, Istanbul, Turkey aut NATIONALLICENCE 773 0- Surgery Today Springer-Verlag 30/5(2000-05-01), 421-425 0941-1291 30:5<421 2000 30 595 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer