caa a22 4500 47575381X CHVBK 20180406123532.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s005950050616 doi (NATIONALLICENCE)springer-10.1007/s005950050616 Enhanced graft healing of high-porosity expanded polytetrafluoroethylene grafts by covalent bonding of fibronectin [Elektronische Daten] [Toshiya Nishibe, Yasuhiro Okuda, Toshihiko Kumada, Tatsuzo Tanabe, Keishu Yasuda] The effect of covalent bonding of fibronectin on the patency and graft healing of high-porosity expanded polytetrafluoroethylene (ePTFE) grafts was evaluated. Bilateral carotid grafting was performed in ten mongrel dogs using high-porosity (60μm) ePTFE grafts, 4 cm in length and 4mm in internal diameter, that either had been pretreated by the covalent bonding of fibronectin (fibronectin grafts) or were untreated (control grafts). The grafts were harvested 4 to 6 weeks after surgery and subjected to macroscopic and lightmicroscopic observations. There was no significant difference in patency between the fibronectin grafts and the control grafts with rates of 80% and 70%, respectively. The thrombusfree area score was significantly greater in the fibronectin grafts than in the control grafts, at 86.9% vs 34.0%. Furthermore, the pseudointima was better replaced by fibrous tissue in the fibronectin grafts than in the control grafts, being lined with a layer of endothelial-like cells. More transmural tissue ingrowth was evident in the fibronectin grafts than in the control grafts. The covalent bonding of fibronectin improves graft healing by stimulating transmural tissue ingrowth in high-porosity ePTFE grafts. Springer-Verlag, 2000 covalent bonding of fibronectin nationallicence high-porosity expanded polytetrafluoroethylene graft nationallicence Nishibe Toshiya Department of Cardiovascular Surgery, Hokkaido University School of Medicine, K14, N5, Kita-ku, 060-8648, Sapporo, Japan aut Okuda Yasuhiro Vascular Graft Research Center Co., Ltd., 1-16-3 Kitakasai, Edogawa-ku, Tokyo, Japan aut Kumada Toshihiko Vascular Graft Research Center Co., Ltd., 1-16-3 Kitakasai, Edogawa-ku, Tokyo, Japan aut Tanabe Tatsuzo Sapporo Hospital of Higashi Nippon Telegraph and Telephone Corp., M1, N15, Chuo-ku, Sapporo, Japan aut Yasuda Keishu Department of Cardiovascular Surgery, Hokkaido University School of Medicine, K14, N5, Kita-ku, 060-8648, Sapporo, Japan aut Surgery Today Springer-Verlag 30/5(2000-05-01), 426-431 0941-1291 30:5<426 2000 30 595 https://doi.org/10.1007/s005950050616 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950050616 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishibe Toshiya Department of Cardiovascular Surgery, Hokkaido University School of Medicine, K14, N5, Kita-ku, 060-8648, Sapporo, Japan aut NATIONALLICENCE 700 1- Okuda Yasuhiro Vascular Graft Research Center Co., Ltd., 1-16-3 Kitakasai, Edogawa-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Kumada Toshihiko Vascular Graft Research Center Co., Ltd., 1-16-3 Kitakasai, Edogawa-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Tanabe Tatsuzo Sapporo Hospital of Higashi Nippon Telegraph and Telephone Corp., M1, N15, Chuo-ku, Sapporo, Japan aut NATIONALLICENCE 700 1- Yasuda Keishu Department of Cardiovascular Surgery, Hokkaido University School of Medicine, K14, N5, Kita-ku, 060-8648, Sapporo, Japan aut NATIONALLICENCE 773 0- Surgery Today Springer-Verlag 30/5(2000-05-01), 426-431 0941-1291 30:5<426 2000 30 595 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer