caa a22 4500 475754239 CHVBK 20180406123533.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s005950070020 doi (NATIONALLICENCE)springer-10.1007/s005950070020 Injury to the Gastric Mucosa and Cellular Dynamics in a Rat Model of Duodenogastric Reflux: The Possible Significance of Gastrin Induction and a Heat Shock Protein [Elektronische Daten] [Nobuhiro Kubo, Tsuyoshi Noguchi, Shinsuke Takeno, Kazuhide Tohara, Yuzo Uchida, Hiroshi Shimoda] Injury to the gastric mucosa caused by duodenogastric reflux (DGR) is often encountered after gastrectomy or truncal vagotomy (V) with pyloroplasty. This study was designed to investigate the histological features of the gastric mucosa under such conditions. A rat model of DGR and DGR+V was established and the thickness of the oxyntic mucosa was measured. Cellular dynamics in the presence of injury to the gastric mucosa caused by DGR were investigated by the immunohistochemical staining of bromodeoxyuridine (BrdU) and heat shock protein 70 (HSP70). The relationship between persistent hypergastrinemia and mucosal injury was also studied. Duodenogastric reflux activated the intracellular induction of HSP70 in our rat model of DGR. Hypergastrinemia was noted in the V group. Compared with values from the DGR group, the numbers of BrdU-labeled cells increased, the glandular proliferation zone expanded, and the thickness of the oxyntic mucosa was significantly higher in the DGR+V group. Compared with the DGR group, there was greater induction of HSP in the DGR+V group during the acute stage. This finding suggests that denervation of the gastric mucosa and hypergastrinemia after vagotomy may be associated with the expression of HSP. Springer-Verlag, 2000 Key words Gastric mucosal injury nationallicence Duodenogastric reflux nationallicence Heat shock protein 70 nationallicence Hypergastrinemia nationallicence Cytoprotection nationallicence Kubo Nobuhiro Departments of Surgery II and aut Noguchi Tsuyoshi Departments of Surgery II and aut Takeno Shinsuke Departments of Surgery II and aut Tohara Kazuhide Departments of Surgery II and aut Uchida Yuzo Departments of Surgery II and aut Shimoda Hiroshi Anatomy I, Oita Medical University, 1-1 Idaigaoka, Hasama-machi, Oita 879-5593, Japan, JP aut https://doi.org/10.1007/s005950070020 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005950070020 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kubo Nobuhiro Departments of Surgery II and aut NATIONALLICENCE 700 1- Noguchi Tsuyoshi Departments of Surgery II and aut NATIONALLICENCE 700 1- Takeno Shinsuke Departments of Surgery II and aut NATIONALLICENCE 700 1- Tohara Kazuhide Departments of Surgery II and aut NATIONALLICENCE 700 1- Uchida Yuzo Departments of Surgery II and aut NATIONALLICENCE 700 1- Shimoda Hiroshi Anatomy I, Oita Medical University, 1-1 Idaigaoka, Hasama-machi, Oita 879-5593, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer