caa a22 4500 475762657 CHVBK 20180406123556.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s005800070023 doi (NATIONALLICENCE)springer-10.1007/s005800070023 Erythropoiesis in the Diploid and Tetraploid Odontophrynus americanus [Elektronische Daten] An Evolutionary Approach in These Cryptic Species (Amphibia, Anura, Leptodactylidae) [A. M. Cianciarullo, M. J. Soares, W. Beçak] Abstract:: Polyploidy is one of Nature's strategies to create diversity among fauna and flora, resulting in new species. We have used light, scanning and transmission electron microscopy to perform morphometric analyses in maturing erythroid cells of the cryptic species diploid and tetraploid Odontophrynus americanus frogs. Normal blood of both specimens contained 97%-99% erythrocytes and 1%-3% reticulocytes, besides thrombocytes and leucocytes. Mature erythrocytes were flattened, ellipsoidal, nucleated, with cytoplasm rich in haemoglobin. Five days after being made anaemic, 15% and 33% of diploid and tetraploid red blood cells respectively, were in an immature stage, basically proerythroblasts. These cells were also seen at the 10th day, in addition to basophilic and polychromatophilic erythroblasts. By day 15 a higher number, 75% and 89% of reticulocytes in earlier maturation stage was found, respectively. At day 20 of recovery from anaemia, there were 63% and 85% of reticulocytes, respectively, most in an advanced stage of maturation. The number of immature cells then gradually decreased at days 30 and 50. Cytoplasmic inclusions similar to Heinz bodies were found in these cells associated with RNA or RNP. Morphometric analysis showed that the tetraploid erythroid cells synthesise 30% more ribosomes than the diploid erythroid cells. The density of ribosomes/μm2 allowed these cells to be classified into seven classes: proerythroblasts, basophilic erythroblasts I and II, polychromatophilic erythroblasts I and II, reticulocytes and erythrocytes. Such morphometric strategy suggested that gene activity was more intense in the tetraploid maturing erythroid cells, despite the marked tendency of these tetraploid cells towards diploidisation of the genome expression. Springer-Verlag London Limited, 2000 Key words:Amphibian evolution - Cryptic species - Erythropoiesis - Gene regulation - Heinz bodies - Morphometric analyses -Odontophrynus americanus- Polyploidy - Prosomes - RNA nationallicence Cianciarullo A. M. Laboratório de Genética, Instituto Butantan, São Paulo, Brasil, BR aut Soares M. J. Departmento de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brasil, BR aut Beçak W. Laboratório de Genética, Instituto Butantan, São Paulo, Brasil, BR aut https://doi.org/10.1007/s005800070023 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005800070023 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cianciarullo A. M. Laboratório de Genética, Instituto Butantan, São Paulo, Brasil, BR aut NATIONALLICENCE 700 1- Soares M. J. Departmento de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brasil, BR aut NATIONALLICENCE 700 1- Beçak W. Laboratório de Genética, Instituto Butantan, São Paulo, Brasil, BR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer