caa a22 4500 475762711 CHVBK 20180406123556.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s005800070026 doi (NATIONALLICENCE)springer-10.1007/s005800070026 Haemopoietic Regeneration After Autohaemotransplant in Sublethal X-Irradiated Marbled Electric Rays [Elektronische Daten] [A. Pica, L. Cristino, F. S. Sasso, P. Guerriero] Abstract:: Haemopoiesis of Elasmobranchs provides a useful model for haematopoietic studies owing to the compartmentalisation of various maturative lineages in spleen (erythropoiesis and lymphocytopoiesis), oesophageal wall and epigonal tissue (myelopoiesis and lymphocytopoiesis). The survival capacity and the recovery of peripheral blood cells and haemopoietic organs were studied in Torpedo marmorata Risso subjected to different single doses of total-body X-ray and following autologous haemotransplantation. Torpedoes were found to be very radioresistant animals from the high dose (100 Gy) of X-ray able to produce complete destruction of haematopoietic tissues but no immediate lethality. Here we report a successful autologous peripheral blood transplantation following 100 Gy X-ray that produces death of the control torpedoes from failure of haemopoiesis. The recovery of the circulating red blood cells after autohaemotransfusion was completed in 28 days, whereas that of the white cells was slower. On day 28 after autohaemotransplant the animals displayed recovery of the haemopoietic tissues, as demonstrated by the BUdR method, but irradiated controls displayed no blood cell or haemopoietic regeneration. Springer-Verlag London Limited, 2000 Key words:Autohaemotransplant - Blood - Fish - Haemopoietic organs - Torpedoes - X-irradiation nationallicence Pica A. Department of Evolutionary and Comparative Biology, University of Naples ‘Federico II', IT aut Cristino L. Department of Evolutionary and Comparative Biology, University of Naples ‘Federico II', IT aut Sasso F. S. Institute of Radiological Sciences of Faculty of Medicine, II University of Naples, Italy, IT aut Guerriero P. Institute of Radiological Sciences of Faculty of Medicine, II University of Naples, Italy, IT aut https://doi.org/10.1007/s005800070026 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005800070026 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pica A. Department of Evolutionary and Comparative Biology, University of Naples ‘Federico II', IT aut NATIONALLICENCE 700 1- Cristino L. Department of Evolutionary and Comparative Biology, University of Naples ‘Federico II', IT aut NATIONALLICENCE 700 1- Sasso F. S. Institute of Radiological Sciences of Faculty of Medicine, II University of Naples, Italy, IT aut NATIONALLICENCE 700 1- Guerriero P. Institute of Radiological Sciences of Faculty of Medicine, II University of Naples, Italy, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer