caa a22 4500 475762797 CHVBK 20180406123556.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s005800070005 doi (NATIONALLICENCE)springer-10.1007/s005800070005 Comparative Nuclear Magnetic Resonance Studies of Diffusional Water Permeability of Red Blood Cells from Different Species. XI. Horses Introduced to Australia and European Horses ( Equus caballus ) [Elektronische Daten] [Gh. Benga, H. Matei, L. Frenţescu, B. E. Chapman, P. W. Kuchel] Abstract:: The diffusional water permeability (P d) of red blood cells (RBCs) from horses introduced to Australia and from European horses was measured by a Mn2+ doping nuclear magnetic resonance (NMR) technique. The values of P d were ∼3.5 × 10−3 cm/s at 25°C, 4.1 × 10−3 cm/s at 30°C, 5.6 × 10−3 cm/s at 37°C and 6.3 × 10−3 cm/s at 42°C with no significant differences between the two strains of horse. Systematic studies on the effect of p-chloromercuribenzene sulphonate (PCMBS) on water diffusion indicated that the maximal inhibition (60%) was reached in 60 min at 22°C with 1 mm PCMBS. The basal permeability to water was estimated at 1.2 × 10−3 cm/s at 25°C, 1.7 × 10−3 cm/s at 30°C, 2.0 × 10−3 cm/s at 37°C and 2.7 × 10−3 cm/s at 42°C. The activation energy (E a,d) of water diffusion was ∼25 kJ/mol and increased to ∼39 kJ/mol after incubation with PCMBS, in conditions of maximal inhibition of water diffusion. The membrane protein electrophoretic pattern of horse RBCs was compared with its human counterpart. The horse RBC membranes had lower amounts of the proteins migrating as bands 4.1 and 4.2 and higher amounts of the protein migrating as band 4.9, and band 6 (glyceraldehyde-3-phosphate dehydrogenase) was undetectable. A noteworthy feature was the appearance of considerable differences in protein migration distances in the region of bands 7 and 9, between horse and human membranes. Springer-Verlag London Limited, 2000 Key words:Aquaporin - Horse - NMR - Red blood cells - Water permeability nationallicence Benga Gh Department of Cell and Molecular Biology, ‘Iuliu Haţieganu' University of Medicine and Pharmacy, Romania, Romania aut Matei H. Department of Cell and Molecular Biology, ‘Iuliu Haţieganu' University of Medicine and Pharmacy, Romania, Romania aut Frenţescu L. Department of Cell and Molecular Biology, ‘Iuliu Haţieganu' University of Medicine and Pharmacy, Romania, Romania aut Chapman B. E. Department of Biochemistry, University of Sydney, NSW, Australia, Australia aut Kuchel P. W. Department of Biochemistry, University of Sydney, NSW, Australia, Australia aut Comparative Haematology International Springer-Verlag 10/3(2000-12-01), 138-143 0938-7714 10:3<138 2000 10 580 https://doi.org/10.1007/s005800070005 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005800070005 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Benga Gh Department of Cell and Molecular Biology, ‘Iuliu Haţieganu' University of Medicine and Pharmacy, Romania, Romania aut NATIONALLICENCE 700 1- Matei H. Department of Cell and Molecular Biology, ‘Iuliu Haţieganu' University of Medicine and Pharmacy, Romania, Romania aut NATIONALLICENCE 700 1- Frenţescu L. Department of Cell and Molecular Biology, ‘Iuliu Haţieganu' University of Medicine and Pharmacy, Romania, Romania aut NATIONALLICENCE 700 1- Chapman B. E. Department of Biochemistry, University of Sydney, NSW, Australia, Australia aut NATIONALLICENCE 700 1- Kuchel P. W. Department of Biochemistry, University of Sydney, NSW, Australia, Australia aut NATIONALLICENCE 773 0- Comparative Haematology International Springer-Verlag 10/3(2000-12-01), 138-143 0938-7714 10:3<138 2000 10 580 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer