caa a22 4500 475762924 CHVBK 20180406123556.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s005800070016 doi (NATIONALLICENCE)springer-10.1007/s005800070016 Differential Analysis of Rat Bone Marrow by Flow Cytometry [Elektronische Daten] [A. Saad, M. Palm, S. Widell, S. Reiland] Abstract:: A procedure for rat bone marrow differential analysis using flow cytometry and commercially available monoclonal antibodies is described. The method uses a combination of the differential expression of leucocyte common antigen (CD45) on different cell lineages and the expression of transferrin receptor (CD71). This is coupled with the side scatter measurement (SSC) for morphological complexity and nucleic acid staining with LDS-751 for the separation of all nucleated cells from mature red blood cells. The relative number of erythroid, myeloid and lymphoid cells as well as the M:E ratio was generated by the flow cytometric method with acceptable reproducibility and a good agreement with microscopic analysis. There were no significant differences in mean values of the three cell populations between males and females or between bone marrow samples collected from left and right femurs. The flow cytometric analysis of bone marrow provides a semi-automated and rapid method that is suitable for the evaluation of rat bone marrow in the preclinical toxicity investigations. Springer-Verlag London Limited, 2000 Key words: Bone marrow nationallicence CD45 nationallicence CD71 nationallicence Flow cytometry nationallicence Rat nationallicence Saad A. AstraZeneca R&D, Safety Assessment, Södertälje, Sweden, SE aut Palm M. AstraZeneca R&D, Safety Assessment, Södertälje, Sweden, SE aut Widell S. Department of Medicine and Department of Oncology and Pathology, Karolinska Hospital and Institute, Stockholm, Sweden, SE aut Reiland S. AstraZeneca R&D, Safety Assessment, Södertälje, Sweden, SE aut https://doi.org/10.1007/s005800070016 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s005800070016 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saad A. AstraZeneca R&D, Safety Assessment, Södertälje, Sweden, SE aut NATIONALLICENCE 700 1- Palm M. AstraZeneca R&D, Safety Assessment, Södertälje, Sweden, SE aut NATIONALLICENCE 700 1- Widell S. Department of Medicine and Department of Oncology and Pathology, Karolinska Hospital and Institute, Stockholm, Sweden, SE aut NATIONALLICENCE 700 1- Reiland S. AstraZeneca R&D, Safety Assessment, Södertälje, Sweden, SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer