caa a22 4500 475763351 CHVBK 20180406123557.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s003960050011 doi (NATIONALLICENCE)springer-10.1007/s003960050011 Novel polyaminoacidic copolymers as nonviral gene vectors [Elektronische Daten] [G. Giammona, G. Cavallaro, G. Pitarresi, E. Pedone]  Human gene therapy is one of the most promising methods developed in recent years, providing great potential for the treatment of a variety of diseases. Complexes formed between DNA and cationic polymers are attracting increasing attention as novel synthetic vectors for the delivery of genes. We have synthesized polycations with quaternary ammonium groups in their side chains for self-assembly with calf thymus DNA. This paper describes the functionalization of α,β-polyasparthydrazide (PAHy), a synthetic macromolecule having many potential applications in the field of biomedical sciences, with glycidyltrimethylammonuim chloride (GTA) in order to introduce positive charges into their chains. Derivatized PAHy with various GTA contents have been obtained and characterized. Highly functionalized copolymers have been used for condensing DNA, yielding discrete complexes. The complex formation has been confirmed by gel electrophoresis and the surface charge of interpolyelectrolyte complexes has been assessed by the zeta potential. Springer-Verlag Berlin Heidelberg, 2000 Key words α,β-Polyasparthydrazide nationallicence Gene therapy nationallicence DNA complexes nationallicence Gene delivery nationallicence Giammona G. Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, I-90123 Palermo Italy e-mail: gaegiamm@mbox.unipa.it Tel.: +39-91-6236128 Fax: +39-91-6177333, IT aut Cavallaro G. Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, I-90123 Palermo Italy e-mail: gaegiamm@mbox.unipa.it Tel.: +39-91-6236128 Fax: +39-91-6177333, IT aut Pitarresi G. Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, I-90123 Palermo Italy e-mail: gaegiamm@mbox.unipa.it Tel.: +39-91-6236128 Fax: +39-91-6177333, IT aut Pedone E. Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, I-90123 Palermo Italy e-mail: gaegiamm@mbox.unipa.it Tel.: +39-91-6236128 Fax: +39-91-6177333, IT aut https://doi.org/10.1007/s003960050011 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s003960050011 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Giammona G. Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, I-90123 Palermo Italy e-mail: gaegiamm@mbox.unipa.it Tel.: +39-91-6236128 Fax: +39-91-6177333, IT aut NATIONALLICENCE 700 1- Cavallaro G. Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, I-90123 Palermo Italy e-mail: gaegiamm@mbox.unipa.it Tel.: +39-91-6236128 Fax: +39-91-6177333, IT aut NATIONALLICENCE 700 1- Pitarresi G. Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, I-90123 Palermo Italy e-mail: gaegiamm@mbox.unipa.it Tel.: +39-91-6236128 Fax: +39-91-6177333, IT aut NATIONALLICENCE 700 1- Pedone E. Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, I-90123 Palermo Italy e-mail: gaegiamm@mbox.unipa.it Tel.: +39-91-6236128 Fax: +39-91-6177333, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer