caa a22 4500 475763424 CHVBK 20180406123558.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s003960050014 doi (NATIONALLICENCE)springer-10.1007/s003960050014 Depolymerization of hyaluronate by the phototoxic drugs phenothiazines and sulfacetamide [Elektronische Daten] [T. Miyazaki, C. Yomota, S. Okada] Phenothiazines (promazine, promethazine, chlorpromazine) and sulfacetamide, known as phototoxic drugs, depolymerize aqueous sodium hyaluronate (HA) on exposure to light. The reduction in the HA molecular weight was followed by size-exclusion chromatography with low-angle laser light scattering. In the low-concentration region of the drugs below 0.05 mM, the rate constants of depolymerization increased. The molecular weight of HA was practically unchanged without UV irradiation in the presence of drugs or with UV irradiation in the absence of drugs, indicating the phenothiazines and sulfacetamide require photoenergy to yield any kind of damaging chemical species for HA depolymerization. An involvement of active oxygen radicals in the effects of promazine and promethazine was evidenced by inhibition under anaerobic conditions. Further, addition of mannitol controlled the reaction in the presence of oxygen, pointing to hydroxyl radicals as the damaging agent. Chlorpromazine and sulfacetamide preferably depolymerized HA under anaerobic conditions, suggesting the participation of hydrated electrons. Springer-Verlag Berlin Heidelberg, 2000 Key words Hyaluronate nationallicence Depolymerization nationallicence Phenothiazines nationallicence Sulfacetamide nationallicence UV irradiation nationallicence Miyazaki T. National Institute of Health Sciences, Osaka 1-1-43 Hoenzaka, Chuo-ku Osaka 540, Japan e-mail: miyazaki@nihs.go.jp Tel.: +81-6-69411533 Fax: +81-6-69420716, JP aut Yomota C. National Institute of Health Sciences, Osaka 1-1-43 Hoenzaka, Chuo-ku Osaka 540, Japan e-mail: miyazaki@nihs.go.jp Tel.: +81-6-69411533 Fax: +81-6-69420716, JP aut Okada S. National Institute of Health Sciences, Osaka 1-1-43 Hoenzaka, Chuo-ku Osaka 540, Japan e-mail: miyazaki@nihs.go.jp Tel.: +81-6-69411533 Fax: +81-6-69420716, JP aut https://doi.org/10.1007/s003960050014 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s003960050014 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miyazaki T. National Institute of Health Sciences, Osaka 1-1-43 Hoenzaka, Chuo-ku Osaka 540, Japan e-mail: miyazaki@nihs.go.jp Tel.: +81-6-69411533 Fax: +81-6-69420716, JP aut NATIONALLICENCE 700 1- Yomota C. National Institute of Health Sciences, Osaka 1-1-43 Hoenzaka, Chuo-ku Osaka 540, Japan e-mail: miyazaki@nihs.go.jp Tel.: +81-6-69411533 Fax: +81-6-69420716, JP aut NATIONALLICENCE 700 1- Okada S. National Institute of Health Sciences, Osaka 1-1-43 Hoenzaka, Chuo-ku Osaka 540, Japan e-mail: miyazaki@nihs.go.jp Tel.: +81-6-69411533 Fax: +81-6-69420716, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer