caa a22 4500 47576370X CHVBK 20180406123558.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s003960000351 doi (NATIONALLICENCE)springer-10.1007/s003960000351 X-ray photoelectron spectroscopy as a tool for studies of the surface layer of microspheres. The case of polystyrene and poly(styrene-acrolein) microspheres with attached human serum albumin [Elektronische Daten] [S. Slomkowski, D. Kowalczyk, M. M. Chehimi, M. Dealamar] The application of X-ray photoelectron spectroscopy (XPS) for studies of surface layers of objects with spherical shape was investigated using as examples polystyrene and poly(styrene-acrolein) microspheres with attached human serum albumin (HSA). The amounts of immobilized protein were determined by the standard biochemical Lowry method and by XPS, using the intensity of the N1s signals of HSA as a basis for evaluation. The XPS data were treated by taking into account the spherical shape of the particles analyzed (variable take-off angle of ejected electrons). The best agreement between the results of the biochemical and XPS determinations was found assuming that for the average particle the takeoff angle varies from 0° to 72.7°. This reflects the fact that in the multilayer arrangement of particles, placed onto the support of the XPS apparatus, the particles from the upper layer partially screen the edges of the particles in the layer below. Springer-Verlag Berlin Heidelberg, 2000 Key words X-ray photoelectron spectroscopy nationallicence Variable takeoff angle nationallicence Polystyrene microspheres nationallicence Poly(styrene nationallicence acrolein) microspheres nationallicence Human serum albumin nationallicence Slomkowski S. Center of Molecular and Macromolecular Studies Polish Academy of Sciences Sienkiewicza 112, 90-363 Lodz, Poland e-mail: staslomk@bilbo.cbmm.lodz.pl Tel.: +48-42-6819815 Fax: +48-42-6847126, PL aut Kowalczyk D. Center of Molecular and Macromolecular Studies Polish Academy of Sciences Sienkiewicza 112, 90-363 Lodz, Poland e-mail: staslomk@bilbo.cbmm.lodz.pl Tel.: +48-42-6819815 Fax: +48-42-6847126, PL aut Chehimi M. M. Institut de Topologie et de Dynamique des Systèmes Université Paris 7 - CNRS (URA 34) 1 rue Guy de la Brosse, 75005 Paris France, FR aut Dealamar M. Institut de Topologie et de Dynamique des Systèmes Université Paris 7 - CNRS (URA 34) 1 rue Guy de la Brosse, 75005 Paris France, FR aut https://doi.org/10.1007/s003960000351 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s003960000351 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Slomkowski S. Center of Molecular and Macromolecular Studies Polish Academy of Sciences Sienkiewicza 112, 90-363 Lodz, Poland e-mail: staslomk@bilbo.cbmm.lodz.pl Tel.: +48-42-6819815 Fax: +48-42-6847126, PL aut NATIONALLICENCE 700 1- Kowalczyk D. Center of Molecular and Macromolecular Studies Polish Academy of Sciences Sienkiewicza 112, 90-363 Lodz, Poland e-mail: staslomk@bilbo.cbmm.lodz.pl Tel.: +48-42-6819815 Fax: +48-42-6847126, PL aut NATIONALLICENCE 700 1- Chehimi M. M. Institut de Topologie et de Dynamique des Systèmes Université Paris 7 - CNRS (URA 34) 1 rue Guy de la Brosse, 75005 Paris France, FR aut NATIONALLICENCE 700 1- Dealamar M. Institut de Topologie et de Dynamique des Systèmes Université Paris 7 - CNRS (URA 34) 1 rue Guy de la Brosse, 75005 Paris France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer