caa a22 4500 475763742 CHVBK 20180406123558.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s003960000335 doi (NATIONALLICENCE)springer-10.1007/s003960000335 Study on size growth in the vesicle formation upon detergent removal from phospholipid/ detergent mixed micelles [Elektronische Daten] [C. Sun, M. Ueno] When 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS) was removed from the mixed CHAPS/EggPC micelles, large vesicles were prepared by dialysis or by slow step-by-step dilution, but small vesicles were prepared by fast one-step dilution. When sodium cholate was removed from the sodium cholate/EggPC micelles, small vesicles formed either by dialysis or by dilution; however, in the presence of 5 mM Ca2+ large vesicles were produced by dialysis, while small vesicles were prepared by dilution. The size growth was related to a detergent-induced fusion of the vesicles containing a large amount of detergent. Using spectrophotometry, quasielastic light scattering and freeze-fracture electron microscopy the fusion events were investigated both through the process of vesicle solubilization by adding detergent and through the process of vesicle formation by diluting a mixed micelle. The results suggest that a rapid CHAPS-induced fusion of the vesicles led to the large resultant vesicles and that no fusion of vesicles containing sodium cholate is responsible for the formation of small vesicles. Furthermore, the ultimate vesicle size related to rapid or slow detergent removal is dependent on the kinetic aspects of the fusion. Springer-Verlag Berlin Heidelberg, 2000 Key words Vesicle formation nationallicence Detergent removal nationallicence Size growth nationallicence Detergent-induced fusion nationallicence Different dilutions nationallicence Sun C. Faculty of Pharmaceutical Sciences Toyama Medical and Pharmaceutical University, 2630 Sugitani Toyama 930-0194, Japan e-mail: mueno@ms.toyama-mpu.ac.jp Tel.: +81-76-434-7565 Fax: +81-76-434-5050, JP aut Ueno M. Faculty of Pharmaceutical Sciences Toyama Medical and Pharmaceutical University, 2630 Sugitani Toyama 930-0194, Japan e-mail: mueno@ms.toyama-mpu.ac.jp Tel.: +81-76-434-7565 Fax: +81-76-434-5050, JP aut https://doi.org/10.1007/s003960000335 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s003960000335 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun C. Faculty of Pharmaceutical Sciences Toyama Medical and Pharmaceutical University, 2630 Sugitani Toyama 930-0194, Japan e-mail: mueno@ms.toyama-mpu.ac.jp Tel.: +81-76-434-7565 Fax: +81-76-434-5050, JP aut NATIONALLICENCE 700 1- Ueno M. Faculty of Pharmaceutical Sciences Toyama Medical and Pharmaceutical University, 2630 Sugitani Toyama 930-0194, Japan e-mail: mueno@ms.toyama-mpu.ac.jp Tel.: +81-76-434-7565 Fax: +81-76-434-5050, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer