caa a22 4500 475763777 CHVBK 20180406123559.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s003960000354 doi (NATIONALLICENCE)springer-10.1007/s003960000354 Effects of charge density and hydrophobicity of ionene polymer on cell binding and viability [Elektronische Daten] [T. Narita, R. Ohtakeyama, M. Nishino, J. P. Gong, Y. Osada] The binding of cationic ionenes onto budding protoplast was investigated and the results were associated with the cell viability. There are critical numbers of carbon atoms to induce effective cell disruption and cell binding. The longer the alkyl chain of the ionene, the lower the concentration at which cell disruption occurs. The ionenes with increased charge density undergo effective binding, while almost 2 orders of magnitude higher concentration are required for effective cell disruption. These results were associated with the cooperativity of the binding process, which induces local stress and solubilization of the lipid membrane. Springer-Verlag Berlin Heidelberg, 2000 Key words Yeast protoplast nationallicence Membrane disruption nationallicence Cooperativity nationallicence Ionene polymer nationallicence Narita T. Division of Biological Sciences Graduate School of Science Hokkaido University Sapporo 060-0810, Japan e-mail: osada@sci.hokudai.ac.jp, JP aut Ohtakeyama R. Division of Biological Sciences Graduate School of Science Hokkaido University Sapporo 060-0810, Japan e-mail: osada@sci.hokudai.ac.jp, JP aut Nishino M. Division of Biological Sciences Graduate School of Science Hokkaido University Sapporo 060-0810, Japan e-mail: osada@sci.hokudai.ac.jp, JP aut Gong J. P. Division of Biological Sciences Graduate School of Science Hokkaido University Sapporo 060-0810, Japan e-mail: osada@sci.hokudai.ac.jp, JP aut Osada Y. Division of Biological Sciences Graduate School of Science Hokkaido University Sapporo 060-0810, Japan e-mail: osada@sci.hokudai.ac.jp, JP aut https://doi.org/10.1007/s003960000354 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s003960000354 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Narita T. Division of Biological Sciences Graduate School of Science Hokkaido University Sapporo 060-0810, Japan e-mail: osada@sci.hokudai.ac.jp, JP aut NATIONALLICENCE 700 1- Ohtakeyama R. Division of Biological Sciences Graduate School of Science Hokkaido University Sapporo 060-0810, Japan e-mail: osada@sci.hokudai.ac.jp, JP aut NATIONALLICENCE 700 1- Nishino M. Division of Biological Sciences Graduate School of Science Hokkaido University Sapporo 060-0810, Japan e-mail: osada@sci.hokudai.ac.jp, JP aut NATIONALLICENCE 700 1- Gong J. P. Division of Biological Sciences Graduate School of Science Hokkaido University Sapporo 060-0810, Japan e-mail: osada@sci.hokudai.ac.jp, JP aut NATIONALLICENCE 700 1- Osada Y. Division of Biological Sciences Graduate School of Science Hokkaido University Sapporo 060-0810, Japan e-mail: osada@sci.hokudai.ac.jp, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer